| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Boykin, Alissa (7)
|
VATAC |
24.42 |
27.13 |
2.71 |
| 2 |
Aquino, Meagan E (7)
|
VAODAC |
28.78 |
27.50 |
-1.28 |
| 3 |
Galiotos, Sophia J (7)
|
VATIDE |
27.72 |
28.94 |
1.22 |
| 4 |
Eberly, Marin C (7)
|
VATIDE |
26.00 |
30.29 |
4.29 |
| 5 |
Masson, Alina G (7)
|
VATIDE |
29.80 |
30.46 |
0.66 |
| 6 |
Metcalf, Annalyn M (7)
|
VAODAC |
|
33.78 |
- |
| 7 |
Guzman, Maite A (7)
|
VATIDE |
35.94 |
36.19 |
0.25 |
| 8 |
Edwards, Sadie E (7)
|
VATAC |
39.44 |
38.78 |
-0.66 |
| 9 |
Eberle, Lisa M (7)
|
VATIDE |
40.25 |
40.97 |
0.72 |
| 10 |
Derrick, Lacey V (7)
|
VATIDE |
36.22 |
41.72 |
5.50 |
|
Krell, Riley A (7)
|
VAECAT |
|
DQ |
- |
|
Heustis, Bailey M (7)
|
VAODAC |
|
DQ |
- |
|
Patterson, Madelynn A (7)
|
VATIDE |
|
DQ |
- |
|
Poteran, Leadon E (7)
|
VATIDE |
|
NS |
- |