| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Owens, Lauryn M (7)
|
VATIDE |
32.28 |
24.84 |
-7.44 |
| 2 |
Swartzbaugh, Anna M (7)
|
VAODAC |
19.75 |
26.84 |
7.09 |
| 3 |
Bodniewicz, Diana A (7)
|
VAODAC |
30.72 |
30.37 |
-0.35 |
| 4 |
Johnson, Chloe M (7)
|
VATIDE |
|
32.37 |
- |
| 5 |
Hartley, Sophie A (7)
|
VATIDE |
|
33.68 |
- |
| 6 |
Davila-Ramos, Ayrabelle (7)
|
VAODAC |
36.41 |
35.06 |
-1.35 |
| 7 |
Washington, Saniyah M (7)
|
VATIDE |
|
38.41 |
- |
| 8 |
Frye, Kenley A (7)
|
VATIDE |
|
40.55 |
- |
| 9 |
Carroll, Emma C (7)
|
VAODAC |
44.28 |
46.16 |
1.88 |
|
Robinson, Angelina L (7)
|
VAODAC |
39.22 |
NS |
- |
|
Latta, Parker L (7)
|
VATIDE |
|
NS |
- |