| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kohler, Olivia A (7)
|
VATAC |
32.84 |
32.58 |
-0.26 |
| 2 |
Dietrich, Stella R (7)
|
VATIDE |
32.29 |
32.90 |
0.61 |
| 3 |
Galiotos, Sophia J (7)
|
VATIDE |
33.66 |
34.69 |
1.03 |
| 4 |
Eberly, Marin C (7)
|
VATIDE |
|
36.40 |
- |
| 5 |
Edwards, Sadie E (7)
|
VATAC |
39.73 |
37.83 |
-1.90 |
| 6 |
Aquino, Meagan E (7)
|
VAODAC |
|
38.28 |
- |
| 7 |
Derrick, Lacey V (7)
|
VATIDE |
|
46.66 |
- |
|
Novak, Liliana E (7)
|
VADIG |
|
DQ |
- |
|
Slivinski, Maeryn L (7)
|
VAODAC |
|
DQ |
- |
|
Vigilance, Riley S (7)
|
VAODAC |
|
DQ |
- |
|
Culicerto, June C (7)
|
VATIDE |
|
DQ |
- |
|
Eberle, Lisa M (7)
|
VATIDE |
|
DQ |
- |
|
Maisenbacher, Elise K (7)
|
VATIDE |
|
DQ |
- |
|
Masson, Alina G (7)
|
VATIDE |
|
DQ |
- |
|
Krell, Riley A (7)
|
VAECAT |
|
NS |
- |
|
Leasure, Alynn R (7)
|
VATAC |
|
NS |
- |