| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nance, Madelyn P (6)
|
VACGBD |
|
22.66 |
- |
| 2 |
Stimson, Lily M (6)
|
VADIG |
29.05 |
25.68 |
-3.37 |
| 3 |
Maciejewski, Mckinley K (6)
|
VACGBD |
|
26.57 |
- |
| 4 |
Shean, May E (5)
|
VAODAC |
29.74 |
27.00 |
-2.74 |
| 5 |
Wilkerson, Charlotte A (6)
|
VATIDE |
28.61 |
28.26 |
-0.35 |
| 6 |
Kubik, Alina R (6)
|
VAODAC |
31.53 |
28.47 |
-3.06 |
| 7 |
Hartley, Thea J (6)
|
VATIDE |
26.06 |
28.75 |
2.69 |
| 8 |
Wieck, Cailey J (6)
|
VAODAC |
27.46 |
28.78 |
1.32 |
| 9 |
Johnson, Brynn E (6)
|
VATAC |
35.94 |
32.81 |
-3.13 |
| 10 |
Barlow, Jade E (6)
|
VACGBD |
31.35 |
34.22 |
2.87 |
| 11 |
Stimson, Mia Y (4)
|
VADIG |
42.85 |
35.57 |
-7.28 |
| 12 |
Miller, Elizabeth A (6)
|
VATAC |
|
38.01 |
- |
| 13 |
Watson, Anne W (5)
|
VATIDE |
|
38.64 |
- |
| 14 |
Masson, Mira C (5)
|
VATIDE |
|
40.25 |
- |
| 15 |
Bonfiglio, Leah T (6)
|
VATIDE |
|
41.40 |
- |
| 16 |
Foxworth, Katherine E (6)
|
VAODAC |
42.28 |
43.35 |
1.07 |
| 17 |
Brashear, Sidney E (6)
|
VATIDE |
|
43.47 |
- |
| 18 |
Sprague, Julianne G (5)
|
VATIDE |
|
44.43 |
- |
| 19 |
Gill, Darby N (5)
|
VATIDE |
|
46.26 |
- |