| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Duey, Adelaide R (6)
|
VATIDE |
49.31 |
28.34 |
-20.97 |
| 2 |
Taylor, Olivia L (6)
|
VATIDE |
20.62 |
29.35 |
8.73 |
| 3 |
Slivinski, Maeryn L (6)
|
VAODAC |
27.09 |
31.25 |
4.16 |
| 4 |
Cutchins, Elizabeth D (5)
|
VATIDE |
|
36.94 |
- |
| 5 |
Johnson, Brynn E (5)
|
VATAC |
38.22 |
39.10 |
0.88 |
| 6 |
Kubik, Emily C (5)
|
VAODAC |
40.15 |
39.75 |
-0.40 |
| 7 |
Wilkerson, Charlotte A (6)
|
VATIDE |
36.55 |
40.38 |
3.83 |
| 8 |
Bonfiglio, Leah T (6)
|
VATIDE |
40.40 |
46.69 |
6.29 |
| 9 |
Korte, Mary D (5)
|
VATIDE |
|
47.10 |
- |
| 10 |
Barnes, Presley A (5)
|
VAODAC |
43.41 |
48.00 |
4.59 |
| 11 |
Watson, Anne W (5)
|
VATIDE |
|
49.10 |
- |
| 12 |
Masson, Mira C (5)
|
VATIDE |
37.26 |
51.09 |
13.83 |
| 13 |
Foxworth, Katherine E (6)
|
VAODAC |
42.28 |
54.22 |
11.94 |
| 14 |
Haywood, Simone G (5)
|
VAECAT |
48.16 |
1:00.60 |
12.44 |
|
Kubik, Alina R (6)
|
VAODAC |
35.85 |
DQ |
- |