| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bowman, Avery M (10)
|
OHPHST |
33.90 |
32.68 |
-1.22 |
| 2 |
Johnson, August D (10)
|
OHBKYS |
36.01 |
34.44 |
-1.57 |
| 3 |
Bollinger, Asher T (9)
|
OHBKYS |
37.11 |
34.60 |
-2.51 |
| 4 |
Bollinger, Beckett A (9)
|
OHBKYS |
36.41 |
36.36 |
-0.05 |
| 4 |
May, Isabella C (10)
|
OHPHST |
38.01 |
36.36 |
-1.65 |
| 6 |
Bennett, Genevieve G (9)
|
OHUAC |
36.30 |
38.07 |
1.77 |
| 7 |
Keskar, Sara (9)
|
OHBKYS |
46.18 |
39.73 |
-6.45 |
| 8 |
Dinovo, Olivia S (9)
|
OHPHST |
44.00 |
41.71 |
-2.29 |
| 9 |
Hoffman, Grace H (8)
|
OHBKYS |
49.13 |
43.51 |
-5.62 |
| 10 |
Riedel, Kayla E (9)
|
OHBKYS |
47.56 |
43.96 |
-3.60 |
| 11 |
Hoffman, Ellie (8)
|
OHBKYS |
46.83 |
44.42 |
-2.41 |
| 12 |
Johnson, Harris W (8)
|
OHBKYS |
46.84 |
45.25 |
-1.59 |
| 13 |
Chapman, Emmeline R (8)
|
OHUAC |
45.00 |
46.09 |
1.09 |
| 14 |
Morfing, Madeline A (8)
|
OHUAC |
57.97 |
46.53 |
-11.44 |
| 15 |
Abner, Arik J (8)
|
OHUAC |
51.31 |
46.97 |
-4.34 |
| 16 |
Giusto, Giuseppe (10)
|
OHBKYS |
|
47.87 |
- |
| 17 |
Drummond, Josephine E (7)
|
OHUAC |
|
49.41 |
- |
| 18 |
Keskar, Sanvi (9)
|
OHBKYS |
54.13 |
49.55 |
-4.58 |
| 19 |
Bernard, Sam A (8)
|
OHBKYS |
1:00.93 |
51.10 |
-9.83 |
| 20 |
Cairns, Eliza (10)
|
OHBKYS |
1:00.49 |
52.54 |
-7.95 |
| 21 |
Li, Jessica Y (10)
|
OHBKYS |
48.00 |
52.93 |
4.93 |
| 22 |
Aldrink, Caroline (9)
|
OHBKYS |
40.99 |
53.53 |
12.54 |
| 23 |
Hoffman, Quinn (5)
|
OHBKYS |
1:05.00 |
58.78 |
-6.22 |
| 24 |
Sieloff, Harper L (8)
|
OHUAC |
54.86 |
1:00.96 |
6.10 |
| 25 |
Navarro Buyanova, Isabel (6)
|
OHBKYS |
|
1:01.17 |
- |
| 26 |
Abner, Astra L (7)
|
OHUAC |
|
1:02.64 |
- |
| 27 |
Botzman, Benjamin C (7)
|
OHUAC |
|
1:19.32 |
- |
| 28 |
Pema, Joshua C (6)
|
OHUAC |
|
1:53.26 |
- |
|
Schiming, Dempsey J (7)
|
OHDCST |
1:00.19 |
DQ |
- |
|
Weir, Edward (10)
|
OHBKYS |
1:05.09 |
NS |
- |
|
Hollander, Moshe Y (9)
|
OHUAC |
|
NS |
- |