| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Arnholt, Wesley H (14)
|
OHPHST |
55.16 |
54.71 |
-0.45 |
| 2 |
Dutton, Ava R (13)
|
OHPHST |
56.10 |
56.34 |
0.24 |
| 3 |
Fortney, Ayden T (13)
|
OHUAC |
1:03.41 |
56.57 |
-6.84 |
| 4 |
Sturgeon, Lydia N (14)
|
OHPHST |
57.27 |
57.07 |
-0.20 |
| 5 |
Jenkins, Matthew D (14)
|
OHPHST |
59.48 |
57.40 |
-2.08 |
| 6 |
Zhu, Ryan S (14)
|
OHLTPY |
1:07.25 |
58.11 |
-9.14 |
| 7 |
Stought, Ryan A (13)
|
OHPHST |
1:01.26 |
1:00.63 |
-0.63 |
| 8 |
Largent, Grace M (13)
|
OHPHST |
1:04.14 |
1:01.89 |
-2.25 |
| 9 |
Grillo Hviid, Annika K (13)
|
OHDCST |
1:05.86 |
1:02.41 |
-3.45 |
| 10 |
Cormican, Hannah E (14)
|
OHPHST |
1:01.81 |
1:02.82 |
1.01 |
| 11 |
Barker, Matthew L (14)
|
OHLTPY |
1:02.45 |
1:03.35 |
0.90 |
| 12 |
Lickfelt, Katie A (14)
|
OHLTPY |
1:05.28 |
1:03.80 |
-1.48 |
| 13 |
Soltanirad, Sara (13)
|
OHBKYS |
1:12.55 |
1:09.95 |
-2.60 |
| 14 |
Wallis, Latham A (13)
|
OHBKYS |
1:16.23 |
1:11.68 |
-4.55 |
| 15 |
Yee, Sydney L (13)
|
OHLTPY |
1:16.68 |
1:12.55 |
-4.13 |
| 16 |
Rateria, Meera (13)
|
OHBKYS |
1:21.50 |
1:16.91 |
-4.59 |
| 17 |
Jenkins, Micah W (13)
|
OHPHST |
1:23.66 |
1:17.53 |
-6.13 |
|
Ginsburg, Lucia R (14)
|
OHLTPY |
1:13.53 |
NS |
- |
|
Remlinger, Abigail J (14)
|
OHPHST |
1:03.61 |
NS |
- |