| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rank, Luciano M (11)
|
NINEWW |
38.65 |
35.75 |
-2.90 |
| 2 |
Alexander, Nathan R (12)
|
NIJETS |
38.91 |
35.99 |
-2.92 |
| 3 |
Mills, William P (12)
|
NIMETS |
37.65 |
36.43 |
-1.22 |
| 4 |
Miller, Treyse B (11)
|
NIMETS |
39.99 |
39.95 |
-0.04 |
| 5 |
Nelson, Payton J (12)
|
NIMETS |
41.64 |
40.41 |
-1.23 |
| 6 |
Zens, Lucien M (11)
|
NICSCI |
40.72 |
40.68 |
-0.04 |
| 7 |
Griffin, Caden J (11)
|
NIJETS |
40.74 |
40.95 |
0.21 |
| 8 |
Winter, Brendan M (9)
|
NIWGSC |
48.13 |
47.95 |
-0.18 |
| 9 |
Vidakovic, Max K (12)
|
NISYCH |
49.82 |
48.66 |
-1.16 |
| 10 |
Mekker, Nathan Z (10)
|
NIJETS |
53.19 |
49.73 |
-3.46 |
| 11 |
Clark, Ethan D (10)
|
NIMETS |
48.10 |
50.83 |
2.73 |
| 12 |
Andrews, William J (11)
|
NIMETS |
49.44 |
50.98 |
1.54 |
| 13 |
Klock, Conner G (10)
|
NIJETS |
1:02.03 |
51.65 |
-10.38 |
| 14 |
Nelson, Caleb W (10)
|
NISYCH |
54.18 |
56.52 |
2.34 |
| 15 |
Windey, Tyler B (10)
|
NIMETS |
|
1:00.03 |
- |
| 16 |
Warner, Lucas M (7)
|
NIMETS |
1:02.93 |
1:16.03 |
13.10 |
|
Perry, Kaden J (11)
|
NIMETS |
|
DQ |
- |
|
Patterson, Finnegan R (10)
|
NIJETS |
|
DQ |
- |
|
Stevens, Jonathan P (12)
|
NIJETS |
55.50 |
NS |
- |
|
Murphy II, Robert L (10)
|
NISYCH |
1:04.83 |
NS |
- |
|
Poulsen, Carter D (11)
|
NISYCH |
57.13 |
NS |
- |