| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Suhayda, Amelia E (10)
|
SEWY |
28.45 |
28.73 |
0.28 |
| 2 |
Hodgkinson, Keely (10)
|
INDY |
30.35 |
30.06 |
-0.29 |
| 3 |
Reed, Gracie (10)
|
INDY |
30.05 |
30.14 |
0.09 |
| 4 |
Brenenborg, Elyse A (9)
|
AMRGSY |
30.96 |
30.80 |
-0.16 |
| 5 |
Murawski, Cali (10)
|
SEWY |
31.95 |
31.30 |
-0.65 |
| 6 |
Keisel, Grace (10)
|
SEWY |
32.43 |
31.35 |
-1.08 |
| 7 |
Shultz, Rylee R (9)
|
AMRGSY |
32.12 |
31.65 |
-0.47 |
| 8 |
Olinger, Piper M (10)
|
AMRGSY |
31.87 |
32.16 |
0.29 |
| 9 |
Anderson, Tenley (10)
|
INDY |
32.08 |
32.23 |
0.15 |
| 10 |
Grove, Jordyn (10)
|
INDY |
33.22 |
32.24 |
-0.98 |
| 11 |
Dodge, Elie (9)
|
LWCY |
34.53 |
32.61 |
-1.92 |
| 12 |
Wissinger, Trinity (10)
|
INDY |
33.97 |
32.71 |
-1.26 |
| 13 |
Thomas, Sophia (10)
|
INDY |
34.27 |
32.77 |
-1.50 |
| 14 |
Pelesky, Isabella (10)
|
LVY |
33.06 |
32.85 |
-0.21 |
| 15 |
Keffalas, Maya (9)
|
AMBYAC |
33.49 |
33.14 |
-0.35 |
| 16 |
Lakes, Allison L (10)
|
AMRESY |
33.63 |
33.18 |
-0.45 |
| 16 |
Savannah, Gianna (10)
|
AMBYAC |
34.25 |
33.18 |
-1.07 |
| 18 |
Culp, Adelynn D (10)
|
AMGJY |
34.30 |
34.15 |
-0.15 |
| 19 |
Hutchins, Madeline M (10)
|
AMGYST |
34.57 |
34.20 |
-0.37 |
| 20 |
Bookwalter, Allison G (8)
|
AMRESY |
35.10 |
34.48 |
-0.62 |
| 21 |
Favela, Elle M (9)
|
AMSPY |
33.77 |
34.77 |
1.00 |
| 22 |
Shipe, Zayley M (8)
|
AMRGSY |
34.55 |
34.80 |
0.25 |
| 23 |
Turner, Alyssa (8)
|
LWCY |
34.36 |
34.97 |
0.61 |
| 24 |
Van Kleunen, Julia X (9)
|
AMBRY |
35.16 |
36.03 |
0.87 |
| 25 |
Griffith, Elizabeth (9)
|
INDY |
34.88 |
36.91 |
2.03 |