| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sisemore, Wyatt D (6)
|
GUESA |
23.97 |
24.60 |
0.63 |
| 2 |
Bialorucki, Camron J (6)
|
GULIFE |
27.21 |
25.83 |
-1.38 |
| 3 |
Rogojina, Kalvin J (6)
|
GUCFSC |
32.78 |
26.69 |
-6.09 |
| 4 |
Walker, Ryan K (6)
|
GUNEHA |
33.36 |
27.38 |
-5.98 |
| 5 |
Hols, Edvin K (6)
|
GUCFSC |
28.00 |
30.40 |
2.40 |
| 6 |
Niemann, Erik J (6)
|
GUCFSC |
30.39 |
32.06 |
1.67 |
| 7 |
Cassani, Emilio N (6)
|
GUCFSC |
38.37 |
37.06 |
-1.31 |
| 8 |
Reyes-Coronel, Dylan R (5)
|
GUPACK |
41.82 |
43.01 |
1.19 |
| 9 |
Pacoma, Kyle Alexander D (4)
|
GUCFSC |
|
48.81 |
- |
|
Smeltzer, Toby W (5)
|
GUESA |
33.34 |
DQ |
- |
|
Bartels, Hayden W (6)
|
GUESA |
26.89 |
NS |
- |
|
Heavrin, Abel K (5)
|
GUESA |
31.17 |
NS |
- |
|
McCauley, Callen R (5)
|
GUPACK |
|
NS |
- |