| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Surratt, Kaitlyn M (14)
|
GUPACK |
2:21.38 |
2:24.71 |
3.33 |
| 2 |
McWhorter, Rowan L (13)
|
GUTYES |
2:32.25 |
2:26.40 |
-5.85 |
| 3 |
Singh, Isabelle H (14)
|
GUPACK |
2:31.37 |
2:26.78 |
-4.59 |
| 4 |
Spencer, Alexandra J (14)
|
GUPACK |
2:32.18 |
2:27.94 |
-4.24 |
| 5 |
Stepanik, Serenity A (13)
|
GUPACK |
2:27.39 |
2:29.62 |
2.23 |
| 6 |
LaPell, Caitlyn M (13)
|
GUPACK |
2:35.22 |
2:35.08 |
-0.14 |
| 7 |
Smith, Berkeley E (14)
|
GUPACK |
2:38.11 |
2:35.97 |
-2.14 |
| 8 |
Hoover, Zoe M (14)
|
GUPACK |
2:55.32 |
2:36.73 |
-18.59 |
| 9 |
Clayton, Elise K (13)
|
GUBBSC |
|
2:42.57 |
- |
| 10 |
O'Sullivan, Ciara A (13)
|
GUPACK |
|
2:46.43 |
- |
| 11 |
Freeman, Brooke H (13)
|
GUTYES |
|
2:49.51 |
- |
| 12 |
Tran, Elyse C (13)
|
GUPACK |
|
3:06.27 |
- |
| 13 |
Yorkey, Olivia L (13)
|
GUESCA |
3:16.05 |
3:06.91 |
-9.14 |
| 14 |
Rost, Ana S (13)
|
GUPACK |
3:12.22 |
3:10.00 |
-2.22 |