| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fuhr, Avery N (15)
|
GUUN-P |
2:23.13 |
2:29.79 |
6.66 |
| 2 |
Gottfried, Charlotte A (16)
|
GUPACK |
2:36.66 |
2:41.29 |
4.63 |
| 3 |
Tilley, Erin E (16)
|
GUPACK |
|
2:44.31 |
- |
| 4 |
Polito, Naomi C (15)
|
GUPACK |
3:31.32 |
2:47.92 |
-43.40 |
| 5 |
Bould, Chelsea E (16)
|
GUPACK |
3:03.18 |
2:55.49 |
-7.69 |
| 6 |
Wise, Shayna M (17)
|
GUTASC |
2:39.66 |
2:55.83 |
16.17 |
| 7 |
Vanderkooi, Rachel H (17)
|
GUTYES |
3:01.67 |
2:57.81 |
-3.86 |
| 8 |
Abrego, Kaylen J (15)
|
GUPACK |
2:53.03 |
2:57.85 |
4.82 |
| 9 |
Gonzales, Chloe N (15)
|
GUNEHA |
3:03.13 |
3:01.21 |
-1.92 |
| 10 |
Griffin, Kennedy A (15)
|
GUPACK |
3:08.73 |
3:06.83 |
-1.90 |