| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Powell, Jessica B (13)
|
GUPACK |
54.58 |
57.98 |
3.40 |
| 2 |
Singh, Isabelle H (14)
|
GUPACK |
57.38 |
58.00 |
0.62 |
| 3 |
Surratt, Kaitlyn M (14)
|
GUPACK |
57.44 |
58.28 |
0.84 |
| 4 |
Reese, Laura M (13)
|
GUPACE |
57.77 |
59.26 |
1.49 |
| 5 |
Ryan, Alyssa Q (14)
|
GUPACE |
1:00.67 |
1:00.95 |
0.28 |
| 6 |
Pham, Kaley V (14)
|
GUPACK |
59.35 |
1:01.32 |
1.97 |
| 7 |
Griffin, Kennedy A (14)
|
GUPACK |
1:03.63 |
1:04.16 |
0.53 |
| 8 |
O'Sullivan, Ciara A (13)
|
GUPACK |
1:01.36 |
1:04.59 |
3.23 |
| 9 |
Abrego, Kaylen J (14)
|
GUPACK |
1:01.29 |
1:05.17 |
3.88 |
| 10 |
Bourgeois-Solomon, Sovereign (14)
|
GUPACE |
1:04.07 |
1:05.95 |
1.88 |
| 11 |
Grzyb, Kalle E (13)
|
GUPACK |
1:02.63 |
1:06.39 |
3.76 |
| 12 |
Lindsey, Kyla J (14)
|
GUPACE |
1:04.59 |
1:06.82 |
2.23 |
| 13 |
Rost, Ana S (13)
|
GUPACK |
1:10.18 |
1:08.29 |
-1.89 |
| 14 |
Green, Emily E (14)
|
GUPACE |
1:03.16 |
1:08.69 |
5.53 |
| 15 |
Rodriguez, Valerie V (13)
|
GUSST |
1:09.53 |
1:09.06 |
-0.47 |
| 16 |
Lester, Courtney A (13)
|
GUPACK |
1:16.05 |
1:11.16 |
-4.89 |
| 17 |
Garcia, Victoria (13)
|
GUUSA |
1:11.44 |
1:11.82 |
0.38 |
| 18 |
Tran, Elyse C (13)
|
GUPACK |
1:14.42 |
1:14.01 |
-0.41 |
| 19 |
Lodzinski, Grace E (13)
|
GUPACK |
1:24.06 |
1:24.33 |
0.27 |
|
Hunt, Kyla V (14)
|
GUPACK |
1:44.66 |
NS |
- |