| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Suarez, Cruz L (10)
|
GUVSST |
30.61 |
30.48 |
-0.13 |
| 2 |
Figueredo-Weiser, Lukas (10)
|
GUVSST |
35.66 |
33.07 |
-2.59 |
| 3 |
Armada, Adrian S (10)
|
GUPACK |
36.17 |
35.28 |
-0.89 |
| 4 |
Zhao, Johnathan M (10)
|
GUPACK |
37.37 |
35.74 |
-1.63 |
| 5 |
McNames, Owen J (9)
|
GUPACE |
37.70 |
37.38 |
-0.32 |
| 6 |
Speedie, Adam J (10)
|
GUVSST |
41.39 |
39.03 |
-2.36 |
| 7 |
Shah, Jonathan K (9)
|
GUPACK |
40.21 |
41.08 |
0.87 |
| 8 |
Wright, Matthew S (10)
|
GUPACK |
43.49 |
42.92 |
-0.57 |
| 9 |
Evans, Brandon K (10)
|
GUUSA |
43.24 |
44.08 |
0.84 |
| 10 |
Revelas, Nicholas W (9)
|
GUVSST |
44.87 |
44.64 |
-0.23 |
| 11 |
Kuebler, Ethan J (9)
|
GUPACK |
41.72 |
45.52 |
3.80 |
| 12 |
Zhang, Haoyang A (9)
|
GUPACK |
46.49 |
46.20 |
-0.29 |
| 13 |
Larson-Hendricks, Anders R (10)
|
GUPACK |
53.55 |
46.41 |
-7.14 |
| 14 |
Stansifer, Ty C (9)
|
GUPACK |
50.62 |
47.95 |
-2.67 |
| 15 |
Canovas, William W (10)
|
GUPACK |
46.19 |
50.12 |
3.93 |
| 16 |
Hamm, Preston R (10)
|
GUPACK |
49.11 |
52.36 |
3.25 |
| 17 |
Depew, Connor E (10)
|
GUPACK |
|
59.25 |
- |
| 18 |
Zhu, Harrison L (10)
|
GUPACK |
1:02.20 |
59.89 |
-2.31 |