| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Darnell, Cooper D (17)
|
GUPACK |
21.65 |
22.78 |
1.13 |
| 2 |
Rex, Nicholas A (19)
|
GUTASC |
22.01 |
22.96 |
0.95 |
| 3 |
Mora, Alejandro M (16)
|
GUTYES |
22.85 |
23.02 |
0.17 |
| 4 |
Ber, Ryan C (15)
|
GUPACK |
22.88 |
23.69 |
0.81 |
| 5 |
Saria, Noah D (15)
|
GUTYES |
24.11 |
24.01 |
-0.10 |
| 6 |
Schonhoff, Andrew P (15)
|
GUPACK |
24.31 |
24.27 |
-0.04 |
| 7 |
Imbern, Ezequiel (16)
|
GUTYES |
24.51 |
24.33 |
-0.18 |
| 8 |
Curl, Jake A (15)
|
GUPACK |
24.94 |
24.77 |
-0.17 |
| 9 |
Moore, Carson E (16)
|
GUPACK |
24.44 |
25.09 |
0.65 |
| 10 |
Bearden, James N (15)
|
GUPACK |
|
25.42 |
- |
| 11 |
Lorenzana, Jason D (16)
|
GUTYES |
26.03 |
25.45 |
-0.58 |
| 12 |
Hoover, Dixon M (15)
|
GUTASC |
25.60 |
26.31 |
0.71 |
| 13 |
Pham, Stefan (16)
|
GUESCA |
26.96 |
26.41 |
-0.55 |
| 14 |
Olson, Noah C (16)
|
GUTASC |
25.07 |
26.57 |
1.50 |
| 15 |
Jensen, Carter T (15)
|
GUTASC |
27.32 |
26.77 |
-0.55 |
| 16 |
McWhorter, Alexander G (15)
|
GUTYES |
26.01 |
26.78 |
0.77 |
| 17 |
Lewis, DeWayne T (16)
|
GUPACK |
27.96 |
28.16 |
0.20 |
| 18 |
Taylor, Christopher A (15)
|
GUTASC |
30.06 |
29.52 |
-0.54 |