| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rudd, Conner D (12)
|
ADCPP |
35.81 |
36.11 |
0.30 |
| 2 |
Keller, Brady C (12)
|
ADCPP |
36.40 |
37.04 |
0.64 |
| 3 |
Hook, Gavin M (12)
|
ADCPP |
42.04 |
41.45 |
-0.59 |
| 4 |
Han, Jake J (11)
|
ADCPP |
41.06 |
43.64 |
2.58 |
| 5 |
Lee, Eun Chan (11)
|
ADCPP |
41.11 |
43.87 |
2.76 |
| 6 |
Upton, Matthew T (12)
|
ADCPP |
44.42 |
44.21 |
-0.21 |
| 7 |
Tieckelmann, Connor R (12)
|
ADUN |
43.47 |
44.47 |
1.00 |
| 8 |
Bhave, Advait G (12)
|
ADUN |
47.44 |
45.36 |
-2.08 |
| 9 |
Simmons, Cooper Z (12)
|
ADCPP |
44.67 |
46.20 |
1.53 |
| 10 |
Ahn, Christopher Y (11)
|
ADCPP |
52.09 |
51.74 |
-0.35 |
| 11 |
Nabaoui, Abdullah (11)
|
ADCPP |
1:00.67 |
59.01 |
-1.66 |
| 12 |
Hughes Robillard, Eric M (11)
|
ADCPP |
1:07.22 |
1:08.21 |
0.99 |