| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hiltbrand, Alexander G (15)
|
NIJETS |
23.54 |
23.64 |
0.10 |
| 2 |
Mullen, Daniel R (18)
|
NIMETS |
22.99 |
23.77 |
0.78 |
| 3 |
Ocker, John R (16)
|
NIMETS |
23.95 |
24.35 |
0.40 |
| 4 |
Bushnell, Christopher C (15)
|
NISYCH |
25.94 |
24.54 |
-1.40 |
| 5 |
Richardson, Austin J (18)
|
NIOLSC |
24.35 |
24.60 |
0.25 |
| 6 |
Long, Dylan M (15)
|
NIMETS |
25.41 |
25.05 |
-0.36 |
| 7 |
Carr, Cameron M (15)
|
NISYCH |
24.74 |
25.27 |
0.53 |
| 8 |
Yost, Anthony J (17)
|
NIMETS |
24.70 |
26.56 |
1.86 |
| 9 |
Tarolli, Vincent M (13)
|
NICSCI |
27.51 |
26.83 |
-0.68 |
| 10 |
Wagner, Alexander J (14)
|
NIJETS |
28.25 |
26.94 |
-1.31 |
| 11 |
Busch, Nolan S (15)
|
NIOLSC |
29.57 |
27.05 |
-2.52 |
| 12 |
Riester, Ethan F (14)
|
NISDSC |
31.21 |
28.01 |
-3.20 |
| 13 |
Andrejko, John T (13)
|
NIJETS |
28.67 |
28.25 |
-0.42 |
| 14 |
Fuller, Garrett D (13)
|
NIMETS |
|
29.03 |
- |
| 15 |
Bennett, Dathan A (14)
|
NIMETS |
32.26 |
31.63 |
-0.63 |
| 16 |
Francisco, Ethan R (13)
|
NIMETS |
32.82 |
31.92 |
-0.90 |
| 17 |
Griffin, Andrew R (13)
|
NIJETS |
33.69 |
34.77 |
1.08 |
| 18 |
Jones, Ryley T (14)
|
NIMETS |
|
38.16 |
- |
|
Ignacio, Manu T (13)
|
NIOLSC |
36.34 |
NS |
- |
|
Barrington, Matthew W (13)
|
NICSCI |
29.83 |
NS |
- |