| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Warner, Tyler J (12)
|
NIMETS |
41.63 |
39.16 |
-2.47 |
| 2 |
Carson, Thomas L (12)
|
NISCSD |
39.14 |
40.00 |
0.86 |
| 3 |
Wilson, Ryan (11)
|
NIBSSC |
38.76 |
40.97 |
2.21 |
| 4 |
Liedka, Ryan J (12)
|
NIMETS |
43.04 |
41.22 |
-1.82 |
| 5 |
Burke, Connor D (11)
|
NISYCH |
42.50 |
41.42 |
-1.08 |
| 6 |
Smith, Keenan (12)
|
NIBSSC |
42.07 |
41.56 |
-0.51 |
| 7 |
Medina, Donavan M (11)
|
NISYCH |
43.11 |
42.98 |
-0.13 |
| 8 |
Tonkin, Jack M (12)
|
NIOLSC |
48.38 |
43.74 |
-4.64 |
| 9 |
Balcom, Colby D (12)
|
NIWGSC |
48.55 |
43.77 |
-4.78 |
| 10 |
Kessler, Scott M (12)
|
NIMETS |
44.01 |
43.79 |
-0.22 |
| 11 |
Wales, Nathaniel J (11)
|
NISYCH |
44.06 |
44.10 |
0.04 |
| 12 |
Vinette, Andrew J (12)
|
NIJETS |
45.59 |
46.28 |
0.69 |
| 13 |
Jackson, Graham R (12)
|
NIBSSC |
47.96 |
48.70 |
0.74 |
| 14 |
Anthony, Alan J (11)
|
NIWGSC |
47.86 |
50.86 |
3.00 |
| 15 |
Oakes, Avery D (11)
|
NIMETS |
59.08 |
58.52 |
-0.56 |
| 16 |
Alexander, Nathan R (11)
|
NIJETS |
1:00.89 |
59.61 |
-1.28 |
| 17 |
Gleeson, Liam C (11)
|
NISYCH |
|
1:05.36 |
- |
|
Denkenberger, Quinten M (11)
|
NIOLSC |
|
DQ |
- |
|
Turner, Elijah N (11)
|
NIOLSC |
|
DQ |
- |
|
Finnerty, Sean P (11)
|
NICSCI |
50.37 |
DQ |
- |
|
Andrews, Alec J (11)
|
NIBSSC |
1:05.88 |
DQ |
- |