| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Croke, Ethan M (10)
|
ADADDI |
43.30 |
39.91 |
-3.39 |
| 2 |
Witting, Liam T (9)
|
ADDELM |
45.81 |
42.69 |
-3.12 |
| 3 |
Zelenke, Daniel K (10)
|
ADUSAM |
47.03 |
44.02 |
-3.01 |
| 4 |
McSwieney, Joshua S (9)
|
ADSCHE |
43.68 |
44.91 |
1.23 |
| 5 |
Sulik, Justin R (9)
|
ADALBS |
46.38 |
44.95 |
-1.43 |
| 6 |
Son, Jiho L (9)
|
ADHAWK |
49.47 |
45.64 |
-3.83 |
| 7 |
Cao, James Y (9)
|
ADDELM |
51.26 |
46.08 |
-5.18 |
| 8 |
Wang, Jake H (10)
|
ADSCHE |
45.78 |
47.96 |
2.18 |
| 9 |
Zhong, Yochen (10)
|
ADALBS |
47.48 |
48.42 |
0.94 |
| 10 |
Leanza, Christopher M (10)
|
ADSCHE |
50.88 |
49.53 |
-1.35 |
| 11 |
Tieckelmann, Connor R (10)
|
ADSCHE |
58.60 |
51.26 |
-7.34 |
| 12 |
Roe, Ethan J (10)
|
ADALBS |
52.56 |
53.65 |
1.09 |
| 13 |
Holmes, Sheldon H (10)
|
ADNHA |
1:01.52 |
53.85 |
-7.67 |
| 14 |
Noonan, Patrick D (10)
|
ADDELM |
54.01 |
54.71 |
0.70 |
| 15 |
Murphy, James C (10)
|
ADBENN |
58.04 |
56.14 |
-1.90 |
| 16 |
Stoddart Sybliss, Jonathan L (10)
|
ADHAWK |
51.45 |
57.20 |
5.75 |
| 17 |
Nesbit, Gabriel G (10)
|
ADBENN |
57.84 |
59.52 |
1.68 |
|
Wilson, Tate E (9)
|
ADHAWK |
44.74 |
NS |
- |