| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Roarty, Samantha K (9)
|
ELY |
35.20 |
34.31 |
-0.89 |
| 2 |
Chen, Emily Y (9)
|
MASCAY |
35.25 |
35.00 |
-0.25 |
| 3 |
Wan, Jingyang (9)
|
MASCAY |
37.49 |
37.13 |
-0.36 |
| 4 |
Zettlemoyer, Addison E (9)
|
ELY |
|
38.16 |
- |
| 5 |
Pietraski, Abigail G (10)
|
ELY |
39.02 |
39.22 |
0.20 |
| 6 |
Honn, Natalee (10)
|
MASCAY |
38.84 |
39.31 |
0.47 |
| 7 |
Wafula, Edna (10)
|
MASCAY |
38.74 |
42.09 |
3.35 |
| 8 |
Campbell, Kaitlyn M (10)
|
MASCAY |
44.01 |
42.50 |
-1.51 |
| 9 |
Congiu-Hughes, Chiara (9)
|
MASCAY |
44.22 |
42.97 |
-1.25 |
|
Chen, Abby (10)
|
MASCAY |
42.41 |
NS |
- |
|
Toyokawa, Maya (9)
|
MASCAY |
46.11 |
NS |
- |