| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hartley, Elliana G (10)
|
NIWEBS |
40.19 |
42.53 |
2.34 |
| 2 |
Geary, Maria E (9)
|
NIMERC |
44.31 |
42.66 |
-1.65 |
| 3 |
Chen, Sophie Y (9)
|
NISDSC |
45.17 |
43.45 |
-1.72 |
| 4 |
Maas, Kaylee A (10)
|
NIIRON |
45.34 |
44.62 |
-0.72 |
| 5 |
Cywinski, Anna C (10)
|
NIVICT |
45.70 |
44.65 |
-1.05 |
| 6 |
Zugelder, Shannon (10)
|
NIVELO |
45.97 |
46.87 |
0.90 |
| 7 |
O'Brien, Jolie L (10)
|
NIWEBS |
51.06 |
47.46 |
-3.60 |
| 8 |
Burnham, Madeline (10)
|
NIVELO |
46.25 |
47.86 |
1.61 |
| 9 |
Van Hout, Kennyn A (9)
|
NISDSC |
49.36 |
49.20 |
-0.16 |
| 10 |
Mastrosimone, Nina A (10)
|
NIWEBS |
52.55 |
49.33 |
-3.22 |
| 11 |
Shi, Sasha L (10)
|
NISDSC |
1:02.40 |
51.46 |
-10.94 |
| 12 |
Quinto, Lilian M (9)
|
NIIRON |
52.19 |
51.51 |
-0.68 |
| 13 |
Catalano, Emilee K (10)
|
NIGRAT |
|
51.53 |
- |
| 14 |
Garigen, Natalie K (9)
|
NIWEBS |
57.35 |
51.85 |
-5.50 |
| 15 |
Bogunovic, Leonora (9)
|
NIVELO |
52.62 |
52.03 |
-0.59 |
| 16 |
Magnera, Alaura (10)
|
NIWEBS |
54.82 |
52.10 |
-2.72 |
| 17 |
McGahan, Molly E (9)
|
NIIRON |
54.52 |
52.27 |
-2.25 |
| 18 |
Moroni, Brianna M (9)
|
NIIRON |
1:20.80 |
53.94 |
-26.86 |
| 19 |
Strausser, Cadence A (10)
|
NIGRAT |
55.85 |
55.87 |
0.02 |
| 20 |
Banks, Grace (10)
|
NIVELO |
|
56.93 |
- |
| 21 |
Codjovi, Jelani A (9)
|
NIVELO |
1:03.09 |
58.71 |
-4.38 |
| 22 |
Schaab, Kate (10)
|
NIVELO |
|
1:00.38 |
- |
| 23 |
Chadwick, Eva S (9)
|
NISDSC |
|
1:10.96 |
- |
| 24 |
Galley, Morgan C (10)
|
NIRRST |
1:23.70 |
1:20.18 |
-3.52 |
|
Kappel, Molly C (9)
|
NIVELO |
|
DQ |
- |
|
Garverick, Audrey D (9)
|
NIVELO |
|
DQ |
- |
|
Ayers, Brianna S (9)
|
NIGRSC |
|
DQ |
- |
|
Jattana, Sukhmani K (10)
|
NISDSC |
|
DQ |
- |
|
Thaney, Brigid G (10)
|
NIGRSC |
1:08.31 |
DQ |
- |