| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gilbert, Natalie J (9)
|
NIQUIK |
46.42 |
40.07 |
-6.35 |
| 2 |
Knausz, Eleanor M (10)
|
NISDSC |
42.39 |
40.40 |
-1.99 |
| 3 |
Chen, Chloe Y (10)
|
NISDSC |
52.27 |
44.12 |
-8.15 |
| 4 |
Richards, Sydney R (10)
|
NIGRAT |
47.31 |
44.14 |
-3.17 |
| 5 |
Anthony, Jillian M (10)
|
NIPMSC |
53.86 |
45.96 |
-7.90 |
| 6 |
Resch, Rebecka J (10)
|
NIGRSC |
47.94 |
47.62 |
-0.32 |
| 7 |
Schwandt, Natalie K (10)
|
NIGRAT |
47.96 |
48.52 |
0.56 |
| 8 |
Dinh, Jasmine (10)
|
NIGRSC |
|
53.56 |
- |
| 9 |
Hauber, Riley A (9)
|
NISDSC |
48.79 |
53.59 |
4.80 |
| 10 |
Barber, Cassandra L (10)
|
NIGRSC |
|
55.69 |
- |
| 11 |
Buzzell, Emily H (9)
|
NIGRSC |
53.08 |
56.69 |
3.61 |
| 12 |
Shi, Sasha L (9)
|
NISDSC |
|
1:00.17 |
- |
| 13 |
Thaney, Brigid G (9)
|
NIGRSC |
|
1:03.59 |
- |
|
Zebrak, Madeleine E (9)
|
NISDSC |
|
DQ |
- |
|
Pogroszewski, Frances S (10)
|
NIGCOM |
|
NS |
- |