| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Obuhanych, Zachary J (11)
|
NISDSC |
30.56 |
30.82 |
0.26 |
| 2 |
Kilchevskyy, Michael (11)
|
NIWEBS |
32.26 |
30.86 |
-1.40 |
| 3 |
Lewis, Matthew K (11)
|
NIQUIK |
34.54 |
31.18 |
-3.36 |
| 4 |
Anthony, Jaydon T (12)
|
NIPMSC |
32.81 |
32.52 |
-0.29 |
| 5 |
Bierley, Benjamin R (11)
|
NIWEBS |
33.21 |
33.23 |
0.02 |
| 6 |
Vitale, Christian R (12)
|
NIQUIK |
36.23 |
34.07 |
-2.16 |
| 7 |
Monroe, Lucas J (12)
|
NIPMSC |
36.29 |
34.76 |
-1.53 |
| 8 |
Zornow, Daniel T (11)
|
NIQUIK |
35.25 |
35.10 |
-0.15 |
| 9 |
Cordy, Colin W (12)
|
NISDSC |
|
35.85 |
- |
| 10 |
Fox, Johnathan M (11)
|
NISDSC |
43.00 |
39.28 |
-3.72 |
| 11 |
Cordy, Brandon C (12)
|
NISDSC |
|
39.55 |
- |
| 12 |
Piedigrossi, Christopher J (12)
|
NIGCOM |
43.93 |
40.68 |
-3.25 |
| 13 |
Steele, Alexander J (11)
|
NIWEBS |
44.77 |
44.79 |
0.02 |
| 14 |
Geoca, Andrew K (11)
|
NISDSC |
|
45.79 |
- |
| 15 |
Goltsman, Adam J (11)
|
NISDSC |
52.42 |
46.32 |
-6.10 |
| 16 |
Passinault, Easton J (11)
|
NIGRSC |
45.50 |
46.97 |
1.47 |
| 17 |
Macomber, Tavis D (11)
|
NISDSC |
|
49.45 |
- |
|
Voytynskyy, Petro (12)
|
NIGRSC |
52.46 |
NS |
- |
|
Hedden, Dominick J (11)
|
NIVICT |
41.81 |
NS |
- |
|
Pendri, Vinay R (12)
|
NIGCOM |
42.18 |
NS |
- |
|
Cao, Son T (11)
|
NIGCOM |
|
NS |
- |
|
Blancaflor, Aaron Joseph (11)
|
NIGCOM |
|
NS |
- |