| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Triaszin, Matthew V (15)
|
NIFAST |
57.30 |
57.02 |
-0.28 |
| 2 |
Smith, Oliver S (16)
|
NIWEBS |
1:01.02 |
57.20 |
-3.82 |
| 3 |
Murphy, Brayden (14)
|
NIVICT |
1:00.61 |
59.62 |
-0.99 |
| 4 |
DiBitetto, Jack J (14)
|
NIHAST |
58.96 |
1:00.79 |
1.83 |
| 5 |
Andersen, Christopher M (16)
|
NISDSC |
56.31 |
1:00.88 |
4.57 |
| 6 |
Donato, Timothy P (14)
|
NIUN-F |
59.85 |
1:02.27 |
2.42 |
| 7 |
Turek, Alex J (15)
|
NIVICT |
1:04.45 |
1:02.82 |
-1.63 |
| 8 |
McEwen, Andrew S (17)
|
NIVICT |
1:01.28 |
1:04.36 |
3.08 |
| 9 |
Stolberg, Logan K (18)
|
NIGCOM |
1:05.60 |
1:05.14 |
-0.46 |
| 10 |
Mortimer, Neil F (14)
|
NIVICT |
1:08.12 |
1:05.54 |
-2.58 |
| 11 |
Teator, Jonathan J (17)
|
NISDSC |
1:13.65 |
1:05.57 |
-8.08 |
| 12 |
Riexinger, Colby J (14)
|
NISEAS |
1:06.46 |
1:05.66 |
-0.80 |
| 13 |
Martello, Mitchell J (17)
|
NIVICT |
|
1:06.68 |
- |
| 14 |
Huber, Timothy J (15)
|
NINEWW |
1:08.04 |
1:08.15 |
0.11 |
| 15 |
Rocas, Marcelo (14)
|
NIFAST |
1:10.37 |
1:08.59 |
-1.78 |
| 16 |
Godown, Elijah M (15)
|
NIPMSC |
1:26.57 |
1:16.50 |
-10.07 |
| 17 |
Morreale, Alexander H (13)
|
NIHAST |
1:23.74 |
1:20.64 |
-3.10 |
|
Amthor, Brandon T (17)
|
NISEAS |
55.34 |
NS |
- |
|
Dinh, Benson (13)
|
NIGRSC |
1:11.52 |
NS |
- |