| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Winseman, Maximus M (10)
|
NIVICT |
34.54 |
34.98 |
0.44 |
| 2 |
Golembiewski, Lucas A (10)
|
NIPACK |
40.64 |
37.32 |
-3.32 |
| 3 |
Warren, Wesley C (10)
|
NIWEBS |
48.69 |
43.83 |
-4.86 |
| 4 |
Everett, Benjamin C (10)
|
NIMERC |
46.86 |
47.65 |
0.79 |
| 5 |
Hinds, Benjamin W (9)
|
NIWEBS |
45.14 |
48.83 |
3.69 |
| 6 |
Wakelee, Adam R (9)
|
NIVICT |
53.26 |
49.26 |
-4.00 |
| 7 |
Nowacki, Joshua T (10)
|
NIHAST |
50.79 |
50.80 |
0.01 |
| 8 |
Zappia, Luke H (9)
|
NIPACK |
1:01.08 |
51.78 |
-9.30 |
| 9 |
Urminsky, Maxwell J (9)
|
NISDSC |
46.10 |
54.12 |
8.02 |
| 10 |
Miller, Kevin T (9)
|
NIIRON |
|
56.20 |
- |
| 11 |
Kelley, Gage T (9)
|
NISDSC |
|
1:00.49 |
- |
|
Ellickson, Cameron B (10)
|
NISDSC |
|
DQ |
- |