| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Xu, Ming Na (11)
|
NIFAST |
30.30 |
29.84 |
-0.46 |
| 2 |
Dinh, Nakisha V (11)
|
NIWEBS |
29.74 |
30.14 |
0.40 |
| 3 |
Sandruck, Brynn A (12)
|
NIVICT |
30.40 |
30.96 |
0.56 |
| 4 |
Nguyen, Vivian A (11)
|
NIWEBS |
32.32 |
31.86 |
-0.46 |
| 5 |
Lerner, Lily K (12)
|
NIWEBS |
32.42 |
32.11 |
-0.31 |
| 6 |
DeHollander, Erin K (11)
|
NIVICT |
33.48 |
32.73 |
-0.75 |
| 7 |
Goodridge, Jenna L (11)
|
NIWEBS |
32.73 |
33.20 |
0.47 |
| 8 |
McGraw, Caroline (12)
|
NIRRST |
33.91 |
33.53 |
-0.38 |
| 9 |
Sahin, Melis (12)
|
NIPACK |
33.80 |
33.58 |
-0.22 |
| 10 |
Guagliardo, Giovanna (11)
|
NIIRON |
35.46 |
35.06 |
-0.40 |
| 11 |
Tytler, Candace L (12)
|
NIIRON |
39.15 |
35.34 |
-3.81 |
| 12 |
Romanick, Paige K (12)
|
NIPACK |
34.00 |
35.41 |
1.41 |
| 13 |
Langan, Abigayle R (11)
|
NIVICT |
35.00 |
35.53 |
0.53 |
| 14 |
Bartholomay, Meghan E (11)
|
NIGCOM |
38.97 |
40.11 |
1.14 |
| 15 |
Kazmark, Raelyn J (12)
|
NIVICT |
39.13 |
41.79 |
2.66 |
| 16 |
Carey, Madison H (11)
|
NIIRON |
|
42.44 |
- |
| 17 |
Patrick, Chloe B (12)
|
NIWEBS |
42.29 |
43.69 |
1.40 |
| 18 |
Williams, Sarah E (11)
|
NIHAST |
46.10 |
45.19 |
-0.91 |
| 19 |
Morris, Emlyn O (11)
|
NIPACK |
47.75 |
46.80 |
-0.95 |
| 20 |
Taberski, Maura J (11)
|
NIIRON |
|
47.12 |
- |
| 21 |
Oneil, Isabel D (12)
|
NIWEBS |
42.21 |
47.88 |
5.67 |
| 22 |
Spall, Carlie A (12)
|
NIHAST |
45.13 |
48.19 |
3.06 |
| 23 |
Christodaro, Raena M (12)
|
NIHAST |
49.25 |
48.40 |
-0.85 |
| 24 |
Williams, Tory L (12)
|
NIPACK |
40.29 |
48.55 |
8.26 |
| 25 |
Kazmark, Olivia N (11)
|
NIVICT |
46.65 |
50.16 |
3.51 |
| 26 |
Lamb, Emily A (11)
|
NIMERC |
1:08.41 |
54.10 |
-14.31 |
| 27 |
Lawrence, Taylor B (11)
|
NIFAST |
53.82 |
54.12 |
0.30 |
|
Thoen, Sarah G (11)
|
NISDSC |
1:08.68 |
DQ |
- |
|
Werner, Riley K (12)
|
NIVICT |
1:05.03 |
NS |
- |