| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wong, Taylor (9)
|
NJLIFE |
40.57 |
40.28 |
-0.29 |
| 2 |
Sales, Julia (10)
|
NJSKYY |
44.25 |
41.98 |
-2.27 |
| 3 |
Bacchetta, AnnaMaria (10)
|
NJSKYY |
43.41 |
44.05 |
0.64 |
| 4 |
Benefield, Madison M (10)
|
NJLIFE |
45.83 |
45.04 |
-0.79 |
| 5 |
Rodriguez, Alice (10)
|
NJSKYY |
46.42 |
45.08 |
-1.34 |
| 6 |
Lyons, Olivia G (9)
|
NJSCAR |
53.69 |
48.99 |
-4.70 |
| 7 |
Gunnarsdottir, Kolbrun (10)
|
NJLIFE |
51.04 |
49.32 |
-1.72 |
| 8 |
Verhage, Madison (9)
|
NJSKYY |
58.11 |
52.25 |
-5.86 |
| 9 |
Calderon, Salome (8)
|
NJNJB |
56.33 |
53.56 |
-2.77 |
| 10 |
Jatczyszyn, Allison (10)
|
NJSKYY |
55.58 |
1:00.42 |
4.84 |