| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sylvester-Johnson, Jillian B (11)
|
VAGATR |
33.08 |
33.95 |
0.87 |
| 2 |
White, Jakiah I (11)
|
VASMAC |
35.49 |
37.19 |
1.70 |
| 3 |
DeSantana-Guidry, Khloe (12)
|
VACYAC |
38.67 |
37.38 |
-1.29 |
| 4 |
Abdulaeva, Nicole (12)
|
VASMAC |
|
37.51 |
- |
| 5 |
Miracle, Audrey C (12)
|
VACYAC |
44.50 |
38.13 |
-6.37 |
| 5 |
White, Jayla M (12)
|
VASMAC |
36.74 |
38.13 |
1.39 |
| 7 |
McWhorter, McKenzie M (12)
|
VASMAC |
40.36 |
39.44 |
-0.92 |
| 8 |
Wohlford, Emma T (12)
|
VAGATR |
42.97 |
41.03 |
-1.94 |
| 9 |
Cronk, Erika R (11)
|
VASMAC |
41.29 |
41.05 |
-0.24 |
| 10 |
Munday, Madeleine K (12)
|
VAGATR |
40.68 |
42.10 |
1.42 |
| 11 |
Richardson, Anna K (11)
|
VASMAC |
43.59 |
43.48 |
-0.11 |
| 12 |
Davis, Morgan M (11)
|
VAGATR |
42.15 |
43.66 |
1.51 |
| 13 |
Macklin, Sophia R (12)
|
VAGATR |
43.42 |
44.48 |
1.06 |
| 14 |
Campbell, Bailey E (11)
|
VAVOLT |
45.44 |
45.60 |
0.16 |
|
Cole, Elsie E (12)
|
VASMAC |
|
DQ |
- |
|
Moseley, Kaitlyn S (11)
|
VAGATR |
39.85 |
NS |
- |