| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pullen, Alexis L (14)
|
VASMAC |
29.22 |
29.27 |
0.05 |
| 2 |
Hendricksen, Meredith A (14)
|
VAGATR |
30.68 |
29.97 |
-0.71 |
| 3 |
Heale, Emmeline K (13)
|
VASMAC |
30.93 |
30.71 |
-0.22 |
| 4 |
Mandujano, Liliana I (13)
|
VASMAC |
30.80 |
31.02 |
0.22 |
| 5 |
Hartley, Madeline G (14)
|
VASMAC |
33.79 |
33.77 |
-0.02 |
| 6 |
White, Jaliyah E (13)
|
VASMAC |
31.16 |
34.23 |
3.07 |
| 7 |
Ward, Liberty H (14)
|
VASMAC |
|
35.14 |
- |
| 8 |
Murdock, Emery N (13)
|
VASMAC |
|
36.08 |
- |
| 9 |
Simmers, Addison L (13)
|
VAGATR |
36.51 |
36.27 |
-0.24 |
| 10 |
Murdock, Eden J (13)
|
VASMAC |
53.82 |
38.04 |
-15.78 |
| 11 |
Michael-Lee, Brynna A (14)
|
VAGATR |
37.75 |
38.21 |
0.46 |
|
Nutter, Lillian E (13)
|
VAGATR |
34.85 |
NS |
- |