| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ismailova, Diana (8)
|
VAGATR |
36.86 |
35.97 |
-0.89 |
| 2 |
Vanyo, Sofie N (8)
|
VACYAC |
39.13 |
39.07 |
-0.06 |
| 3 |
Carnevali, Gabriella G (7)
|
VAGATR |
53.27 |
42.18 |
-11.09 |
| 4 |
Sylvester-Johnson, Jillian B (8)
|
VAGATR |
49.99 |
42.34 |
-7.65 |
| 5 |
White, Jakiah I (8)
|
VASMAC |
53.29 |
48.87 |
-4.42 |
| 6 |
Overstreet, Layla R (8)
|
VACCA |
48.23 |
49.55 |
1.32 |
| 7 |
Richardson, Anna K (8)
|
VASMAC |
|
1:00.02 |
- |
| 8 |
Aldridge, Chloe N (8)
|
VASMAC |
|
1:07.09 |
- |
| 9 |
Ward, Lily E (8)
|
VAKFY |
|
1:14.11 |
- |
|
Piller, Avery K (7)
|
VASMAC |
|
DQ |
- |
|
Speicher, Olivia L (8)
|
VASMAC |
|
DQ |
- |
|
Blakeman, Jacquelyn L (8)
|
VAKFY |
|
DQ |
- |
|
Cooper, Amelia C (7)
|
VAGATR |
59.97 |
DQ |
- |
|
Viar, Olivia B (8)
|
VACCA |
|
DQ |
- |
|
Keller, Claire E (8)
|
VAGATR |
|
DQ |
- |
|
Robertson, Ryleigh H (8)
|
VASMAC |
|
NS |
- |
|
Schaaf, Alexis V (8)
|
VAGATR |
45.35 |
NS |
- |
|
Carter, Emmi D (8)
|
VACCA |
57.26 |
NS |
- |