| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ismailova, Diana (8)
|
VAGATR |
44.47 |
43.53 |
-0.94 |
| 2 |
Vanyo, Sofie N (8)
|
VACYAC |
50.55 |
43.58 |
-6.97 |
| 3 |
White, Jakiah I (8)
|
VASMAC |
59.61 |
50.43 |
-9.18 |
| 4 |
Sloat, Skylar D (7)
|
VASMAC |
56.16 |
52.17 |
-3.99 |
| 5 |
Zhu, Emily (8)
|
VACYAC |
54.17 |
53.38 |
-0.79 |
| 6 |
Sylvester-Johnson, Jillian B (8)
|
VAGATR |
58.97 |
54.03 |
-4.94 |
| 7 |
Carnevali, Gabriella G (7)
|
VAGATR |
56.03 |
55.28 |
-0.75 |
| 8 |
Phillips, Lillian E (8)
|
VACYAC |
1:00.22 |
58.96 |
-1.26 |
| 9 |
Cheng, Ashley (7)
|
VAGATR |
|
58.97 |
- |
| 10 |
Aldridge, Chloe N (8)
|
VASMAC |
|
59.17 |
- |
| 11 |
Deeble, Sadie C (7)
|
VAGATR |
|
1:00.78 |
- |
| 12 |
Wise, Latane I (8)
|
VACYAC |
1:04.29 |
1:02.80 |
-1.49 |
| 13 |
Cooper, Amelia C (7)
|
VAGATR |
1:05.86 |
1:04.95 |
-0.91 |
| 14 |
Gao, Angelina (8)
|
VACYAC |
|
1:15.49 |
- |
|
Renick, Madison K (7)
|
VACCA |
|
DQ |
- |
|
Meadows, Emma F (7)
|
VACCA |
|
DQ |
- |
|
Carter, Emmi D (8)
|
VACCA |
58.80 |
NS |
- |
|
Showalter, Tessa L (7)
|
VACYAC |
|
NS |
- |
|
Schaaf, Alexis V (8)
|
VAGATR |
59.41 |
NS |
- |