| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hanstedt, Lucinda E (14)
|
VASTRM |
27.56 |
26.90 |
-0.66 |
| 2 |
Cazenas, Katie (13)
|
PVRY |
26.42 |
27.32 |
0.90 |
| 3 |
Niculescu, Gabriela E (13)
|
VASMAC |
30.54 |
27.33 |
-3.21 |
| 4 |
Johnson, Keira L (14)
|
VAGATR |
30.02 |
28.47 |
-1.55 |
| 5 |
Murie, Rachel H (14)
|
VASMAC |
29.68 |
28.68 |
-1.00 |
| 6 |
Ragsdale, Audrey J (14)
|
VAGATR |
29.83 |
29.07 |
-0.76 |
| 7 |
Pillion, Abby R (13)
|
VAGATR |
28.90 |
29.22 |
0.32 |
| 8 |
Guan, Yaoxia (14)
|
PVRY |
28.65 |
29.30 |
0.65 |
| 9 |
Casey, Olivia K (14)
|
VASTRM |
28.64 |
29.81 |
1.17 |
| 10 |
Elkins, Alice A (14)
|
VASMAC |
|
31.23 |
- |
| 11 |
Dixon, Julia L (13)
|
VAACST |
|
31.88 |
- |
| 12 |
Vernon, Emmaleigh N (14)
|
VAGATR |
32.04 |
32.86 |
0.82 |
| 13 |
Taylor, Amelia M (14)
|
VAGATR |
33.20 |
33.03 |
-0.17 |
| 14 |
Lamm, Kathryn I (13)
|
VAGATR |
36.77 |
36.97 |
0.20 |
|
Browning, Virginia A (13)
|
VAACST |
|
NS |
- |