| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Moody, Claire V (16)
|
VAGATR |
28.21 |
28.58 |
0.37 |
| 2 |
Wagner, Kristen L (17)
|
VASMAC |
27.91 |
29.00 |
1.09 |
| 3 |
Long, Sofia R (17)
|
VA757 |
28.01 |
29.57 |
1.56 |
| 4 |
McCay, Madeleine H (15)
|
VAGATR |
29.08 |
30.03 |
0.95 |
| 5 |
Gusler, Lily K (17)
|
VAGATR |
28.57 |
30.04 |
1.47 |
| 6 |
Arnold, Sarah E (17)
|
VAGATR |
28.92 |
30.36 |
1.44 |
| 7 |
Johnson, Kylie M (17)
|
VA757 |
29.43 |
30.52 |
1.09 |
| 8 |
Lynch, Virginia N (17)
|
VA757 |
29.74 |
30.65 |
0.91 |
| 9 |
Flanagan, Lainey R (16)
|
VA757 |
29.26 |
30.80 |
1.54 |
| 10 |
Wyatt, Elizabeth (16)
|
VAGATR |
30.94 |
31.00 |
0.06 |
| 11 |
Bendall, Elisabeth A (15)
|
VAGATR |
31.83 |
31.46 |
-0.37 |
| 12 |
Kastner, Niamh C (15)
|
VA757 |
30.92 |
31.62 |
0.70 |
| 13 |
Arndt, Olivia D (17)
|
VAGATR |
30.59 |
31.68 |
1.09 |
| 14 |
Johnson, Keira L (16)
|
VAGATR |
32.09 |
32.17 |
0.08 |
| 15 |
Kashatus, Audrey E (16)
|
VAGATR |
31.28 |
32.38 |
1.10 |
| 16 |
Price, Carleigh N (15)
|
VA757 |
31.99 |
33.64 |
1.65 |
| 17 |
Martin, Avery L (15)
|
VA757 |
32.84 |
34.00 |
1.16 |
| 18 |
Pietrowski, Anja J (15)
|
VASMAC |
|
34.71 |
- |
|
Nardone, Stephanie M (20)
|
VAGATR |
29.82 |
NS |
- |