| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hewitt, James A (9)
|
VAGATR |
40.63 |
42.23 |
1.60 |
| 2 |
Suggs DeSorbo, Matthew J (10)
|
VAGATR |
|
42.86 |
- |
| 3 |
Weaber, Anderson E (9)
|
VASMAC |
1:01.24 |
48.04 |
-13.20 |
| 4 |
Oswald, Aiden C (10)
|
VASMAC |
47.36 |
48.07 |
0.71 |
| 5 |
McMahon, Robert W (10)
|
VASMAC |
47.43 |
49.21 |
1.78 |
| 6 |
Straight, Christian B (10)
|
VASMAC |
50.90 |
50.92 |
0.02 |
| 7 |
Malatesta, Nathan (9)
|
VARACE |
1:00.06 |
52.40 |
-7.66 |
| 8 |
He, Quinn T (10)
|
VASMAC |
1:07.77 |
57.52 |
-10.25 |
| 9 |
Fish, Xavier L (10)
|
VASMAC |
1:03.54 |
57.99 |
-5.55 |
| 10 |
Griffin, Samuel M (10)
|
VASMAC |
|
1:00.13 |
- |
| 11 |
McWhorter, Mason M (10)
|
VASMAC |
|
1:06.96 |
- |
| 12 |
Aponte, Arden M (10)
|
VA757 |
|
1:13.58 |
- |