| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hewitt, James A (9)
|
VAGATR |
38.66 |
41.05 |
2.39 |
| 2 |
Suggs DeSorbo, Matthew J (10)
|
VAGATR |
|
44.97 |
- |
| 3 |
Weaber, Anderson E (9)
|
VASMAC |
50.82 |
45.27 |
-5.55 |
| 4 |
McMahon, Robert W (10)
|
VASMAC |
44.78 |
45.83 |
1.05 |
| 5 |
Oswald, Aiden C (10)
|
VASMAC |
48.92 |
48.63 |
-0.29 |
| 6 |
Straight, Christian B (10)
|
VASMAC |
50.26 |
51.64 |
1.38 |
| 7 |
McWhorter, Mason M (10)
|
VASMAC |
54.83 |
51.82 |
-3.01 |
| 8 |
Griffin, Samuel M (10)
|
VASMAC |
|
52.28 |
- |
| 9 |
Fish, Xavier L (10)
|
VASMAC |
55.67 |
54.98 |
-0.69 |
| 10 |
He, Quinn T (10)
|
VASMAC |
57.81 |
1:01.96 |
4.15 |