| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Moran, Aiden T (9)
|
NICLSC |
34.48 |
34.56 |
0.08 |
| 2 |
Hediger, Kobe K (10)
|
NIUN-E |
35.58 |
34.99 |
-0.59 |
| 3 |
Young-Mandiak, Atticus F (10)
|
NISTAR |
37.02 |
36.39 |
-0.63 |
| 4 |
Anderson, Max (9)
|
NIVELO |
36.44 |
36.56 |
0.12 |
| 5 |
Matteson, Cyrus L (10)
|
NISTAR |
37.20 |
36.87 |
-0.33 |
| 6 |
Wakelee, Adam R (10)
|
NIVICT |
39.45 |
37.55 |
-1.90 |
| 7 |
Coen, Mitchell R (9)
|
NISTAR |
41.01 |
39.82 |
-1.19 |
| 8 |
Ray, Gavin M (10)
|
NIVICT |
|
40.11 |
- |
| 9 |
Rudnicki, William S (10)
|
NITWST |
|
40.46 |
- |
| 10 |
Moldenhauer, Thomas C (9)
|
NIEAST |
42.02 |
41.37 |
-0.65 |
| 11 |
Voit, Nicholas (10)
|
NIAMTS |
|
41.89 |
- |
| 12 |
Rogers, Joseph T (9)
|
NISTAR |
|
42.46 |
- |
| 13 |
Kneeland, Noah J (9)
|
NIVELO |
45.36 |
42.84 |
-2.52 |
| 14 |
Reimann, Nathan S (9)
|
NISTAR |
46.17 |
42.92 |
-3.25 |
| 15 |
Gawron, Ian J (9)
|
NICLSC |
46.05 |
43.53 |
-2.52 |
| 16 |
DellaMea, Nicholas A (9)
|
NIVELO |
43.58 |
43.68 |
0.10 |
| 17 |
Nienhaus, Matthew (9)
|
NISHAQ |
42.53 |
43.84 |
1.31 |
| 18 |
Kendall, Alex M (10)
|
NITTSC |
44.65 |
43.87 |
-0.78 |
| 19 |
Fisher, Simon D (10)
|
NISTAR |
41.80 |
44.37 |
2.57 |
| 20 |
Ray, Landon M (9)
|
NIVICT |
|
44.72 |
- |
| 21 |
Shepler, Jack (10)
|
NIVELO |
|
45.02 |
- |
| 22 |
Reukauf, Nathan A (9)
|
NISTAR |
1:00.26 |
46.29 |
-13.97 |
| 23 |
Pirigyi, Ethan J (10)
|
NISTAR |
45.31 |
46.30 |
0.99 |
| 24 |
Wang, Michael (9)
|
NIVELO |
47.06 |
46.42 |
-0.64 |
| 25 |
Powers, Jack N (10)
|
NIVELO |
|
47.81 |
- |
| 26 |
Crowther, Finn L (10)
|
NIVELO |
51.02 |
48.38 |
-2.64 |
| 27 |
Kobielski, Carter E (9)
|
NIVELO |
52.41 |
55.83 |
3.42 |
| 28 |
DellaMea, Marco V (9)
|
NIVELO |
55.46 |
56.43 |
0.97 |
| 29 |
Wisniewski, Jordan R (10)
|
NIRAYS |
1:42.70 |
1:11.27 |
-31.43 |