| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bruzgul, Jaxon R (12)
|
NITWST |
38.87 |
39.95 |
1.08 |
| 2 |
Borden, Jonas O (12)
|
NIBAAC |
40.35 |
40.23 |
-0.12 |
| 3 |
Peyser, Peter M (11)
|
NIBAAC |
43.12 |
40.26 |
-2.86 |
| 4 |
Dean, Conner T (11)
|
NISTAR |
|
42.58 |
- |
| 5 |
Lemmo, Nicholas D (11)
|
NIVELO |
44.83 |
42.63 |
-2.20 |
| 6 |
Wang, Merrick (11)
|
BORC |
|
42.65 |
- |
| 7 |
Zaki, Daniel (11)
|
BORC |
45.86 |
43.31 |
-2.55 |
| 8 |
Eisenmann, Joshua T (11)
|
NISTAR |
46.67 |
43.65 |
-3.02 |
| 9 |
Amer, Sirajedeen F (11)
|
NIVELO |
44.07 |
43.99 |
-0.08 |
| 10 |
DiGiacomo, Dominic J (12)
|
NICLSC |
|
45.06 |
- |
| 11 |
Gatti, Jake D (12)
|
NICLSC |
45.97 |
46.63 |
0.66 |
| 12 |
Spence, Matthew M (11)
|
NISHAQ |
47.98 |
46.77 |
-1.21 |
| 13 |
Coen, Liam C (11)
|
NISTAR |
48.61 |
46.83 |
-1.78 |
| 14 |
Gross, Noah R (12)
|
NISTAR |
|
47.91 |
- |
| 15 |
Meyer, Noah (11)
|
NIVELO |
47.33 |
48.40 |
1.07 |
| 16 |
Szmanda, Cristopher D (11)
|
NIRAYS |
55.03 |
48.45 |
-6.58 |
| 17 |
Coen, Jack D (11)
|
NISTAR |
43.68 |
48.73 |
5.05 |
| 18 |
LaMoy, Hunter P (12)
|
NIRAYS |
50.53 |
49.06 |
-1.47 |
| 19 |
Burke, Ryan (11)
|
NIVELO |
49.76 |
50.41 |
0.65 |
| 20 |
Crowther, Eli T (11)
|
NIVELO |
57.34 |
54.92 |
-2.42 |
| 21 |
Wachowicz, Joseph (11)
|
NITTSC |
|
57.35 |
- |
| 22 |
Littrell, Alexander W (12)
|
NISTAR |
1:03.96 |
57.48 |
-6.48 |
| 23 |
Roberts, Harrison F (11)
|
NITTSC |
1:00.22 |
58.87 |
-1.35 |