| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wagner, Morgan E (10)
|
NIVICT |
41.05 |
35.97 |
-5.08 |
| 2 |
Xia, Vanessa (10)
|
BORC |
40.80 |
39.88 |
-0.92 |
| 3 |
Cywinski, Anna C (10)
|
NIVICT |
43.96 |
40.78 |
-3.18 |
| 4 |
Huntress, Maya V (10)
|
NIBAAC |
|
42.35 |
- |
| 5 |
Roberts, Meredith J (9)
|
NITTSC |
45.90 |
43.36 |
-2.54 |
| 6 |
Rasmusson, Henrietta E (9)
|
NICLSC |
47.91 |
44.82 |
-3.09 |
| 7 |
Golden, Lauren B (9)
|
NIBAAC |
53.19 |
45.30 |
-7.89 |
| 8 |
Murphy, Campbell E (10)
|
NITTSC |
46.56 |
45.37 |
-1.19 |
| 9 |
Burnham, Madeline (9)
|
NIVELO |
45.71 |
45.41 |
-0.30 |
| 10 |
Amer, Sarah F (9)
|
NIVELO |
50.34 |
52.42 |
2.08 |
| 11 |
Loehfelm, Haley E (10)
|
NITWST |
52.19 |
52.98 |
0.79 |
| 12 |
Whitley-Grassi, Ava E (10)
|
NISTAR |
|
53.73 |
- |
| 13 |
Bogunovic, Leonora (9)
|
NIVELO |
1:00.13 |
57.97 |
-2.16 |
| 14 |
Tothero, Madeleine S (10)
|
NISTAR |
56.21 |
58.22 |
2.01 |
| 15 |
Miller, Amanda C (10)
|
NIVICT |
53.18 |
58.32 |
5.14 |
| 16 |
Martineck, Chase M (9)
|
NIVELO |
|
59.12 |
- |
| 17 |
Attuwaybi, Eman (9)
|
NIVELO |
1:08.80 |
1:01.25 |
-7.55 |
| 18 |
Ryan, Isabella A (9)
|
NIVELO |
1:18.37 |
1:03.52 |
-14.85 |
| 19 |
Pisa, Cadence E (10)
|
NIBAAC |
|
1:04.14 |
- |
| 20 |
Adamczyk, Brianna A (9)
|
NICLSC |
1:00.26 |
1:05.75 |
5.49 |
| 21 |
Alessi, Luciana M (9)
|
NIBAAC |
|
1:06.68 |
- |
| 22 |
Wands, Lucille M (10)
|
NIVELO |
1:03.75 |
1:08.98 |
5.23 |
| 23 |
Baginski, Natasha H (9)
|
NIRAYS |
|
1:11.80 |
- |
| 24 |
Washington, Ashanti M (9)
|
NISTAR |
1:27.10 |
1:12.13 |
-14.97 |
| 25 |
Garverick, Audrey D (9)
|
NIVELO |
1:12.82 |
1:12.28 |
-0.54 |
| 26 |
Jodoin, Destiny B (10)
|
NIVICT |
|
1:22.31 |
- |
|
Hutton, Ryan (9)
|
NIVELO |
1:05.29 |
DQ |
- |
|
Scirri, Ava L (10)
|
NITTSC |
|
DQ |
- |
|
Codjovi, Jelani A (9)
|
NIVELO |
|
NS |
- |