| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bruzgul, Jaxon R (12)
|
NITWST |
35.94 |
35.82 |
-0.12 |
| 2 |
Borden, Jonas O (12)
|
NIBAAC |
40.19 |
38.25 |
-1.94 |
| 3 |
Wang, Merrick (11)
|
BORC |
40.92 |
39.15 |
-1.77 |
| 4 |
Dean, Conner T (11)
|
NISTAR |
40.45 |
39.75 |
-0.70 |
| 5 |
Amer, Sirajedeen F (11)
|
NIVELO |
39.88 |
39.81 |
-0.07 |
| 6 |
Peyser, Peter M (11)
|
NIBAAC |
45.50 |
40.23 |
-5.27 |
| 7 |
Lemmo, Nicholas D (11)
|
NIVELO |
46.07 |
43.71 |
-2.36 |
| 8 |
Coen, Liam C (11)
|
NISTAR |
45.15 |
44.75 |
-0.40 |
| 9 |
Spence, Matthew M (11)
|
NISHAQ |
44.14 |
45.30 |
1.16 |
| 10 |
Tong, Benjamin Y (11)
|
BORC |
49.98 |
46.27 |
-3.71 |
| 11 |
Bouton, Robert A (11)
|
NIBAAC |
46.57 |
47.32 |
0.75 |
| 12 |
Hayter, Daniel J (11)
|
NISHAQ |
1:03.11 |
48.52 |
-14.59 |
| 13 |
Endress, Elton P (12)
|
NIUN-T |
|
48.64 |
- |
| 14 |
Gross, Noah R (12)
|
NISTAR |
|
48.82 |
- |
| 15 |
Eisenmann, Joshua T (11)
|
NISTAR |
47.58 |
49.63 |
2.05 |
| 16 |
Littrell, Alexander W (12)
|
NISTAR |
|
51.13 |
- |
| 17 |
Meyer, Noah (11)
|
NIVELO |
55.61 |
52.35 |
-3.26 |
| 18 |
Burke, Ryan (11)
|
NIVELO |
57.40 |
53.52 |
-3.88 |
| 19 |
Gatti, Jake D (12)
|
NICLSC |
54.37 |
53.81 |
-0.56 |
| 20 |
LaMoy, Hunter P (12)
|
NIRAYS |
|
54.02 |
- |
| 21 |
Crowther, Eli T (11)
|
NIVELO |
|
1:06.12 |
- |
|
Szmanda, Cristopher D (11)
|
NIRAYS |
|
DQ |
- |
|
DiGiacomo, Dominic J (12)
|
NICLSC |
44.05 |
DQ |
- |