| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zhou, Jinyi (12)
|
NIUN-D |
38.80 |
37.98 |
-0.82 |
| 2 |
O'Brien, Kevin J (12)
|
NICLSC |
48.77 |
38.44 |
-10.33 |
| 3 |
Peyser, Peter M (12)
|
NICLSC |
40.36 |
38.65 |
-1.71 |
| 4 |
Young-Mandiak, Atticus F (11)
|
NISTAR |
48.06 |
39.57 |
-8.49 |
| 5 |
Hayter, Daniel J (12)
|
NISHAQ |
47.57 |
40.49 |
-7.08 |
| 6 |
Dean, Conner T (12)
|
NISTAR |
46.58 |
40.92 |
-5.66 |
| 7 |
Clark, Adam C (11)
|
AMPWRS |
45.02 |
43.57 |
-1.45 |
| 8 |
Ramos, Logan A (11)
|
NIVELO |
50.18 |
43.97 |
-6.21 |
| 9 |
Coen, Jack D (12)
|
NISTAR |
44.97 |
44.28 |
-0.69 |
| 10 |
Low, Louis (11)
|
BORC |
45.00 |
44.85 |
-0.15 |
| 11 |
Marthi, Pranav C (11)
|
BORC |
45.07 |
45.18 |
0.11 |
| 12 |
Matteson, Cyrus L (11)
|
NISTAR |
45.73 |
45.87 |
0.14 |
| 13 |
Sands, Konrad (12)
|
BORC |
45.00 |
48.74 |
3.74 |
| 14 |
Crowther, Finn L (11)
|
NIVELO |
51.37 |
49.51 |
-1.86 |
| 15 |
Slaughter, Timothy A (12)
|
NIVELO |
54.38 |
50.50 |
-3.88 |
| 16 |
Mendoza, Roberto (12)
|
BORC |
56.14 |
51.27 |
-4.87 |
| 17 |
Roberts, Harrison F (12)
|
NITTSC |
59.51 |
53.64 |
-5.87 |
| 18 |
Shepler, Jack (11)
|
NIVELO |
55.64 |
54.05 |
-1.59 |
| 19 |
Curvin, Sean P (11)
|
NITTSC |
57.08 |
54.07 |
-3.01 |
| 20 |
Olsen, Owen T (11)
|
NIAMTS |
54.31 |
55.33 |
1.02 |
| 21 |
Skoumpris, Joseph P (11)
|
NIVELO |
53.60 |
55.73 |
2.13 |
| 22 |
Kobielski, Colin (12)
|
NIVELO |
1:08.78 |
56.16 |
-12.62 |
| 23 |
Eisenmann, Joshua T (12)
|
NISTAR |
55.72 |
57.34 |
1.62 |
| 24 |
Kendall, Alex M (11)
|
NITTSC |
1:04.20 |
1:03.02 |
-1.18 |
| 25 |
Xu, Jeremy B (11)
|
NIVELO |
|
1:04.00 |
- |
| 26 |
Zuckerberg, Eben M (11)
|
NITTSC |
1:12.64 |
1:04.32 |
-8.32 |
| 27 |
Walter, Jacob P (11)
|
NITTSC |
1:05.16 |
1:05.46 |
0.30 |
| 28 |
Towles-Schwen, Christopher C (11)
|
NIAMTS |
|
1:20.99 |
- |
|
Chelpinski, Alexander (11)
|
NIVELO |
1:14.79 |
DQ |
- |
|
Lorenz, Charles D (11)
|
NITTSC |
52.84 |
NS |
- |