| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Golden, Lauren B (8)
|
NIBAAC |
49.14 |
47.32 |
-1.82 |
| 2 |
Amer, Sarah (8)
|
NIVELO |
55.13 |
51.54 |
-3.59 |
| 3 |
Piscitello, Aniella O (8)
|
NIRAYS |
|
51.93 |
- |
| 4 |
Clouser, Kerrington D (8)
|
NIBCSR |
1:08.86 |
53.01 |
-15.85 |
| 5 |
Burnham, Madeline (8)
|
NIVELO |
53.26 |
53.72 |
0.46 |
| 6 |
Honaker, Addison R (8)
|
NICLSC |
52.93 |
54.97 |
2.04 |
| 7 |
Bogunovic, Leonora (8)
|
NIVELO |
57.51 |
55.58 |
-1.93 |
| 8 |
King, Izzy M (8)
|
NIEAST |
1:04.61 |
56.80 |
-7.81 |
| 9 |
Alessi, Luciana M (8)
|
NIBAAC |
54.38 |
57.43 |
3.05 |
| 10 |
Codjovi, Jelani (8)
|
NIVELO |
|
57.58 |
- |
| 11 |
Jermak, Amelia E (7)
|
NIBAAC |
58.56 |
57.71 |
-0.85 |
| 12 |
Ryan, Isabella A (8)
|
NIVELO |
1:02.86 |
1:00.01 |
-2.85 |
| 13 |
Loehfelm, Madelyn G (7)
|
NITWST |
1:03.57 |
1:00.66 |
-2.91 |
| 14 |
Attuwaybi, Eman (8)
|
NIVELO |
1:00.96 |
1:01.40 |
0.44 |
| 15 |
Schmitt, Kimberly R (8)
|
NITTSC |
1:02.59 |
1:02.46 |
-0.13 |
| 16 |
Fritsch, Lauren P (8)
|
NITTSC |
1:03.04 |
1:06.37 |
3.33 |
| 17 |
Attuwaybi, Hibah (7)
|
NIVELO |
1:04.21 |
1:07.86 |
3.65 |
| 18 |
Umansky, Katarina R (7)
|
NIBAAC |
1:00.18 |
1:08.23 |
8.05 |
| 19 |
Griffin, Grace K (8)
|
NITTSC |
1:16.03 |
1:09.62 |
-6.41 |
| 20 |
Hufnagel, Megan K (6)
|
NITTSC |
1:12.96 |
1:13.13 |
0.17 |
| 21 |
Marcyan, Reese J (7)
|
NITTSC |
1:10.03 |
1:13.37 |
3.34 |
| 22 |
Baker, Catherine M (8)
|
NITWST |
|
1:13.92 |
- |
| 23 |
Washington, Ashanti M (8)
|
NISTAR |
1:15.56 |
1:18.93 |
3.37 |