| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Golden, Lauren B (8)
|
NIBAAC |
42.45 |
41.56 |
-0.89 |
| 2 |
Amer, Sarah (8)
|
NIVELO |
42.82 |
42.78 |
-0.04 |
| 3 |
Roberts, Meredith J (8)
|
NITTSC |
47.13 |
45.75 |
-1.38 |
| 4 |
Clouser, Kerrington D (8)
|
NIBCSR |
54.76 |
46.81 |
-7.95 |
| 5 |
Alessi, Luciana M (8)
|
NIBAAC |
46.59 |
47.27 |
0.68 |
| 6 |
Attuwaybi, Eman (8)
|
NIVELO |
50.62 |
47.39 |
-3.23 |
| 7 |
Maussner, Melia S (8)
|
NISTAR |
46.52 |
48.04 |
1.52 |
| 8 |
Burnham, Madeline (8)
|
NIVELO |
48.65 |
49.30 |
0.65 |
| 9 |
Ryan, Isabella A (8)
|
NIVELO |
50.17 |
49.43 |
-0.74 |
| 10 |
Bogunovic, Leonora (8)
|
NIVELO |
47.87 |
49.66 |
1.79 |
| 11 |
Codjovi, Jelani (8)
|
NIVELO |
|
49.97 |
- |
| 12 |
King, Izzy M (8)
|
NIEAST |
50.29 |
51.00 |
0.71 |
| 13 |
Washington, Ashanti M (8)
|
NISTAR |
55.03 |
54.54 |
-0.49 |
| 14 |
Umansky, Katarina R (7)
|
NIBAAC |
55.83 |
54.89 |
-0.94 |
| 15 |
Attuwaybi, Hibah (7)
|
NIVELO |
50.99 |
55.33 |
4.34 |
| 16 |
Jermak, Amelia E (7)
|
NIBAAC |
56.42 |
58.41 |
1.99 |
| 17 |
Loehfelm, Madelyn G (7)
|
NITWST |
55.00 |
58.52 |
3.52 |
| 18 |
Griffin, Grace K (8)
|
NITTSC |
1:03.20 |
1:01.75 |
-1.45 |
| 19 |
Hufnagel, Megan K (6)
|
NITTSC |
1:04.25 |
1:03.79 |
-0.46 |
| 20 |
Marcyan, Reese J (7)
|
NITTSC |
1:02.66 |
1:05.04 |
2.38 |
|
Bhat, Maryam M (6)
|
NIBAAC |
1:20.26 |
NS |
- |