| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Waldron, Keller D (12)
|
NITTSC |
42.70 |
39.82 |
-2.88 |
| 2 |
Peyser, Peter M (11)
|
NIBAAC |
40.26 |
39.86 |
-0.40 |
| 3 |
El Ghissassi, Awab (11)
|
NIVEST |
38.64 |
40.18 |
1.54 |
| 4 |
Borden, Jonas O (12)
|
NIBAAC |
40.23 |
40.37 |
0.14 |
| 5 |
Wang, Merrick (11)
|
BORC |
42.65 |
41.03 |
-1.62 |
| 6 |
Dean, Conner T (11)
|
NISTAR |
42.58 |
41.67 |
-0.91 |
| 7 |
LaJeunesse, Peter F (12)
|
NIVELO |
42.80 |
41.76 |
-1.04 |
| 8 |
Tong, Benjamin Y (11)
|
BORC |
46.00 |
41.81 |
-4.19 |
| 9 |
Bruzgul, Jayden D (11)
|
NITWST |
46.61 |
42.29 |
-4.32 |
| 10 |
Lemmo, Nicholas D (11)
|
NIVELO |
42.63 |
42.94 |
0.31 |
| 11 |
Zaki, Daniel (11)
|
BORC |
43.31 |
43.39 |
0.08 |
| 12 |
Madden, Tristen S (11)
|
NIFALC |
42.58 |
43.67 |
1.09 |
| 13 |
Eisenmann, Joshua T (11)
|
NISTAR |
43.65 |
44.55 |
0.90 |
| 14 |
Fan, Justin F (11)
|
NICLSC |
53.45 |
45.76 |
-7.69 |
| 15 |
Coen, Jack D (11)
|
NISTAR |
43.68 |
46.89 |
3.21 |
| 16 |
Gatti, Jake D (12)
|
NICLSC |
45.97 |
47.04 |
1.07 |
| 17 |
Kobielski, Colin (12)
|
NIVELO |
49.62 |
47.50 |
-2.12 |
| 18 |
Coen, Liam C (11)
|
NISTAR |
46.83 |
47.51 |
0.68 |
| 19 |
Burke, Ryan (12)
|
NIVELO |
49.76 |
49.01 |
-0.75 |
| 20 |
Meyer, Noah (11)
|
NIVELO |
47.33 |
49.85 |
2.52 |
| 21 |
Kerr, John M (12)
|
NIVELO |
|
50.40 |
- |
| 22 |
Slaughter, Timothy A (11)
|
NIVELO |
|
51.32 |
- |
| 23 |
Avino, Evan A (12)
|
NISTAR |
52.64 |
51.41 |
-1.23 |
| 24 |
Crowther, Eli T (11)
|
NIVELO |
54.92 |
51.69 |
-3.23 |
| 25 |
LaMoy, Hunter P (12)
|
NIRAYS |
50.53 |
51.75 |
1.22 |
| 26 |
Rider, Benjamin T (12)
|
NIFALC |
|
53.01 |
- |
| 27 |
Littrell, Alexander W (12)
|
NISTAR |
57.48 |
55.21 |
-2.27 |
| 28 |
Sriram, Shyam (12)
|
NIVELO |
54.33 |
56.72 |
2.39 |