| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wagner, Morgan E (10)
|
NIVICT |
2:54.39 |
2:52.28 |
-2.11 |
| 2 |
Reilly, Bridget F (10)
|
NISTAR |
3:24.53 |
3:19.83 |
-4.70 |
| 3 |
Golden, Lauren B (9)
|
NIBAAC |
3:23.46 |
3:21.99 |
-1.47 |
| 4 |
Huntress, Maya V (10)
|
NIBAAC |
|
3:31.12 |
- |
| 5 |
Rasmusson, Henrietta E (9)
|
NICLSC |
4:24.12 |
3:46.23 |
-37.89 |
| 6 |
Burnham, Madeline (9)
|
NIVELO |
3:41.26 |
3:48.15 |
6.89 |
| 7 |
Loehfelm, Haley E (10)
|
NITWST |
3:46.33 |
4:03.04 |
16.71 |
| 8 |
Alessi, Luciana M (9)
|
NIBAAC |
4:04.62 |
4:03.42 |
-1.20 |
| 9 |
Tothero, Madeleine S (10)
|
NISTAR |
4:03.11 |
4:05.10 |
1.99 |
| 10 |
Bogunovic, Leonora (9)
|
NIVELO |
4:05.86 |
4:08.20 |
2.34 |
| 11 |
Wands, Lucille M (10)
|
NIVELO |
4:14.92 |
4:12.09 |
-2.83 |
| 12 |
Codjovi, Jelani A (9)
|
NIVELO |
|
4:13.75 |
- |
| 13 |
Attuwaybi, Eman (9)
|
NIVELO |
4:29.56 |
4:17.27 |
-12.29 |
| 14 |
Jensen, Audrey E (9)
|
NIBAAC |
|
4:19.54 |
- |
| 15 |
Washington, Ashanti M (9)
|
NISTAR |
|
4:54.52 |
- |