| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wagner, Morgan E (10)
|
NIVICT |
35.97 |
34.76 |
-1.21 |
| 2 |
Xia, Vanessa (10)
|
BORC |
39.88 |
37.51 |
-2.37 |
| 3 |
Puzanovova, Veronika A (10)
|
NIBAAC |
48.20 |
42.78 |
-5.42 |
| 4 |
Rasmusson, Henrietta E (9)
|
NICLSC |
44.82 |
46.66 |
1.84 |
| 5 |
Hammons, Milena (10)
|
BORC |
46.70 |
49.32 |
2.62 |
| 6 |
Honaker, Addison R (9)
|
NICLSC |
59.19 |
49.73 |
-9.46 |
| 7 |
Martineck, Chase M (9)
|
NIVELO |
59.12 |
54.06 |
-5.06 |
| 8 |
Baldwin, Rylie L (10)
|
NIUN-N |
|
54.33 |
- |
| 9 |
Jensen, Audrey E (9)
|
NIBAAC |
|
55.00 |
- |
| 10 |
Fritsch, Lauren P (9)
|
NITTSC |
53.44 |
56.53 |
3.09 |
| 11 |
Tothero, Madeleine S (10)
|
NISTAR |
56.21 |
57.37 |
1.16 |
| 12 |
Bogunovic, Leonora (9)
|
NIVELO |
57.97 |
57.48 |
-0.49 |
| 13 |
Griffin, Grace K (9)
|
NITTSC |
|
57.74 |
- |
| 14 |
Alessi, Luciana M (9)
|
NIBAAC |
1:06.68 |
57.96 |
-8.72 |
| 15 |
Muffoletto, Brynn (9)
|
NISHAQ |
1:07.00 |
58.20 |
-8.80 |
| 16 |
Zugelder, Shannon (10)
|
NIVELO |
1:05.74 |
59.66 |
-6.08 |
| 17 |
Gawron, Samantha G (10)
|
NICLSC |
1:01.88 |
1:00.16 |
-1.72 |
| 18 |
Loehfelm, Madelyn G (8)
|
NITWST |
1:06.46 |
1:00.40 |
-6.06 |
| 19 |
Loehfelm, Haley E (10)
|
NITWST |
52.19 |
1:00.73 |
8.54 |
| 20 |
Attuwaybi, Eman (9)
|
NIVELO |
1:01.25 |
1:01.00 |
-0.25 |
| 21 |
Banks, Grace (10)
|
NIVELO |
1:08.55 |
1:02.51 |
-6.04 |
| 22 |
Wands, Lucille M (10)
|
NIVELO |
1:03.75 |
1:05.65 |
1.90 |
| 23 |
Barrera-Hagner, Ananya S (10)
|
NIEAST |
|
1:08.46 |
- |
| 24 |
Baker, Catherine M (9)
|
NITWST |
1:24.17 |
1:12.74 |
-11.43 |
| 25 |
Washington, Ashanti M (9)
|
NISTAR |
1:12.13 |
1:13.74 |
1.61 |
| 26 |
Jodoin, Destiny B (10)
|
NIVICT |
1:22.31 |
1:17.76 |
-4.55 |
|
Burnham, Madeline (9)
|
NIVELO |
45.41 |
DQ |
- |
|
Codjovi, Jelani A (9)
|
NIVELO |
|
DQ |
- |
|
Adamczyk, Brianna A (9)
|
NICLSC |
1:00.26 |
DQ |
- |
|
Schaab, Kate (10)
|
NIVELO |
|
DQ |
- |
|
Haines, Kaylee N (10)
|
NISTAR |
|
DQ |
- |