| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Miao, Alex (12)
|
NIUN-D |
35.00 |
33.20 |
-1.80 |
| 2 |
Vorona, Pavel (12)
|
BORC |
33.82 |
33.22 |
-0.60 |
| 3 |
Peyser, Peter M (11)
|
NIBAAC |
36.70 |
36.57 |
-0.13 |
| 4 |
Borden, Jonas O (12)
|
NIBAAC |
38.25 |
37.82 |
-0.43 |
| 5 |
El Ghissassi, Awab (11)
|
NIVEST |
38.17 |
38.01 |
-0.16 |
| 6 |
Wang, Merrick (11)
|
BORC |
39.15 |
38.62 |
-0.53 |
| 7 |
Priset, Kyle J (12)
|
NICLSC |
|
39.30 |
- |
| 8 |
Dean, Conner T (11)
|
NISTAR |
39.75 |
40.54 |
0.79 |
| 9 |
Lemmo, Nicholas D (11)
|
NIVELO |
43.71 |
42.78 |
-0.93 |
| 10 |
Coen, Jack D (11)
|
NISTAR |
43.62 |
43.93 |
0.31 |
| 11 |
LaJeunesse, Peter F (12)
|
NIVELO |
42.36 |
43.94 |
1.58 |
| 12 |
Coen, Liam C (11)
|
NISTAR |
44.75 |
44.96 |
0.21 |
| 13 |
Zaki, Daniel (11)
|
BORC |
|
45.00 |
- |
| 14 |
Bruzgul, Jayden D (11)
|
NITWST |
43.00 |
45.11 |
2.11 |
| 15 |
Waldron, Keller D (12)
|
NITTSC |
49.60 |
46.37 |
-3.23 |
| 16 |
Eisenmann, Joshua T (11)
|
NISTAR |
47.58 |
46.91 |
-0.67 |
| 17 |
Tong, Benjamin Y (11)
|
BORC |
46.27 |
47.26 |
0.99 |
| 18 |
Madden, Tristen S (11)
|
NIFALC |
|
48.18 |
- |
| 19 |
Gatti, Jake D (12)
|
NICLSC |
53.81 |
53.30 |
-0.51 |
| 20 |
Littrell, Alexander W (12)
|
NISTAR |
51.13 |
53.54 |
2.41 |
| 21 |
Avino, Evan A (12)
|
NISTAR |
|
54.19 |
- |
| 22 |
Kobielski, Colin (12)
|
NIVELO |
1:00.65 |
54.82 |
-5.83 |
| 23 |
Meyer, Noah (11)
|
NIVELO |
52.35 |
55.05 |
2.70 |
| 24 |
Burke, Ryan (12)
|
NIVELO |
53.52 |
56.52 |
3.00 |
| 25 |
LaMoy, Hunter P (12)
|
NIRAYS |
|
59.84 |
- |
| 26 |
Crowther, Eli T (11)
|
NIVELO |
1:06.12 |
1:04.35 |
-1.77 |
| 27 |
Kerr, John M (12)
|
NIVELO |
|
1:11.79 |
- |
|
Slaughter, Timothy A (11)
|
NIVELO |
|
DQ |
- |
|
Sriram, Shyam (12)
|
NIVELO |
1:02.65 |
NS |
- |