| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gao, Victoria G (8)
|
NIWAAC |
27.56 |
25.58 |
-1.98 |
| 2 |
Welliver, Charlotte P (8)
|
NICSST |
|
25.94 |
- |
| 3 |
Henderson, Madeline R (8)
|
NIWAAC |
37.98 |
28.12 |
-9.86 |
| 4 |
Roberts, Olivia M (8)
|
NIHAWK |
39.46 |
37.33 |
-2.13 |
| 5 |
Tyler, Natalie L (7)
|
NICAGO |
|
44.58 |
- |
|
Hagan, Bridget D (8)
|
NICSST |
38.38 |
DQ |
- |
|
Vanderhoof, Emma J (8)
|
NIWAAC |
|
DQ |
- |
|
Leonard, Kaitllyn S (7)
|
NIWAAC |
33.40 |
DQ |
- |
|
Homer, Olivia K (6)
|
NIWAAC |
|
DQ |
- |
|
Denlinger, Elizabeth J (8)
|
NIWAAC |
|
DQ |
- |
|
El Ghissassi, Sidra S (7)
|
NIVEST |
25.06 |
DQ |
- |
|
Wang, Bethany B (5)
|
NIHHTA |
|
DQ |
- |
|
Knowlton, Ava L (8)
|
NIHHTA |
|
DQ |
- |
|
Mika, Madison A (6)
|
NICAGO |
|
DQ |
- |
|
Murphy, Gabriela (8)
|
NIHHTA |
26.73 |
NS |
- |
|
Rought, Elle M (7)
|
NICVSC |
|
NS |
- |
|
Martin-Putman, Ava E (7)
|
NIBEST |
|
NS |
- |