| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Walsh, Teagan M (8)
|
NIHAWK |
30.11 |
29.29 |
-0.82 |
| 2 |
Rollo, Lilyanna E (8)
|
NIUNAC |
|
31.88 |
- |
| 3 |
Clifford, Mikaia M (8)
|
NICORY |
37.05 |
35.20 |
-1.85 |
| 4 |
Jefferis, Frida R (8)
|
NIISWM |
40.07 |
38.96 |
-1.11 |
| 5 |
Chandler, Nora M (7)
|
NICVSC |
|
40.64 |
- |
| 6 |
Roberts, Olivia M (8)
|
NIHAWK |
|
40.92 |
- |
|
Tyler, Natalie L (7)
|
NICVSC |
|
DQ |
- |
|
Reimsnyder, Alyssa L (7)
|
NIHAWK |
|
DQ |
- |
|
Walsh, Brynna M (6)
|
NIHAWK |
|
DQ |
- |
|
Gonzalez, Leah K (7)
|
NIKRKN |
|
DQ |
- |
|
Leahey, Emme A (8)
|
NIUNAC |
|
DQ |
- |
|
Homer, Olivia K (7)
|
NIWAAC |
|
DQ |
- |
|
Spencer, Danielle H (6)
|
NIWAAC |
|
DQ |
- |
|
Fitzwater, Elizabeth O (7)
|
NIGGST |
|
NS |
- |