| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
El Ghissassi, Ritaj (12)
|
NIVEST |
27.69 |
27.72 |
0.03 |
| 2 |
Williams, Taegan S (12)
|
NIWAAC |
28.61 |
29.56 |
0.95 |
| 3 |
Figueroa, Marissa J (12)
|
NICAGO |
29.22 |
29.59 |
0.37 |
| 4 |
Gill-Martiny, Lily M (12)
|
NIUNAC |
29.39 |
29.73 |
0.34 |
| 5 |
Olsian, Ava C (12)
|
NIUNAC |
31.75 |
29.85 |
-1.90 |
| 6 |
Vascoe, Delaney M (12)
|
NIWAAC |
31.14 |
30.10 |
-1.04 |
| 7 |
Stone, Caleigh (11)
|
NICORY |
30.25 |
30.87 |
0.62 |
| 8 |
Bush, Sarah (12)
|
NICAGO |
29.82 |
31.11 |
1.29 |
| 10 |
Marshall, Emily V (11)
|
NIWAAC |
33.40 |
32.81 |
-0.59 |
| 11 |
Headrick, Skyler F (12)
|
NIISWM |
33.13 |
32.91 |
-0.22 |
| 12 |
Sharpsteen, Willow (12)
|
NIWAAC |
32.47 |
32.93 |
0.46 |
| 13 |
Goodreau, Josephine M (12)
|
NIISWM |
34.53 |
33.63 |
-0.90 |
| 14 |
Butler, Malina M (11)
|
NIGGST |
33.48 |
33.99 |
0.51 |
| 15 |
Brandes, Tiernee S (12)
|
NIGVST |
34.58 |
35.05 |
0.47 |
| 16 |
Guo, Angie Y (12)
|
NIISWM |
44.67 |
35.39 |
-9.28 |
| 17 |
Rule, Lilyan M (12)
|
NIUNAC |
36.03 |
35.60 |
-0.43 |
| 18 |
Richeda, Olivia J (11)
|
NIUNAC |
38.83 |
35.69 |
-3.14 |
| 19 |
Romans, Mya O (11)
|
NICORY |
35.84 |
36.76 |
0.92 |
| 20 |
Boyle, Elizabeth E (12)
|
NIWAAC |
|
39.47 |
- |
| 21 |
Adams, Katie E (12)
|
NIGGST |
38.60 |
40.21 |
1.61 |
| 22 |
Michel, Georgia G (11)
|
NIGGST |
40.32 |
41.51 |
1.19 |
| 23 |
Feltz, Taylor A (12)
|
NIKVAC |
47.56 |
41.87 |
-5.69 |
| 24 |
Reese, Reagan a (11)
|
NIGGST |
36.97 |
43.52 |
6.55 |
| 25 |
Marquez, Thali M (11)
|
NIGGST |
39.37 |
43.94 |
4.57 |
| 26 |
Wilson, Brenna M (11)
|
NIKRKN |
|
1:12.98 |
- |
|
Beck, Jenna R (11)
|
NICVSC |
28.84 |
DQ |
- |
|
Callaghan, Kelsey J (12)
|
NIVEST |
|
DQ |
- |
|
Pignatelli, Maria L (12)
|
NIUNAC |
27.14 |
DQ |
- |