| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pignatelli, Maria L (12)
|
NIUNAC |
29.73 |
29.86 |
0.13 |
| 2 |
Williams, Taegan S (12)
|
NIWAAC |
31.85 |
32.91 |
1.06 |
| 3 |
Figueroa, Marissa J (12)
|
NICAGO |
32.32 |
32.92 |
0.60 |
| 4 |
Beck, Jenna R (11)
|
NICVSC |
31.99 |
34.21 |
2.22 |
| 5 |
Vascoe, Delaney M (12)
|
NIWAAC |
35.91 |
34.46 |
-1.45 |
| 7 |
Sharpsteen, Willow (12)
|
NIWAAC |
36.05 |
35.51 |
-0.54 |
| 8 |
Olsian, Ava C (12)
|
NIUNAC |
42.61 |
37.95 |
-4.66 |
| 9 |
Bush, Sarah (12)
|
NICAGO |
37.37 |
38.22 |
0.85 |
| 10 |
Gill-Martiny, Lily M (12)
|
NIUNAC |
40.95 |
38.78 |
-2.17 |
| 11 |
Benton, Alexandra P (12)
|
NIKRKN |
|
39.22 |
- |
| 12 |
Goodreau, Josephine M (12)
|
NIISWM |
37.90 |
39.50 |
1.60 |
| 13 |
Headrick, Skyler F (12)
|
NIISWM |
42.72 |
40.99 |
-1.73 |
| 14 |
Butler, Malina M (11)
|
NIGGST |
38.61 |
41.10 |
2.49 |
| 15 |
Brandes, Tiernee S (12)
|
NIGVST |
44.34 |
41.38 |
-2.96 |
| 16 |
Marshall, Emily V (11)
|
NIWAAC |
46.36 |
41.62 |
-4.74 |
| 17 |
Guo, Angie Y (12)
|
NIISWM |
52.12 |
42.75 |
-9.37 |
| 18 |
Adams, Katie E (12)
|
NIGGST |
44.01 |
42.93 |
-1.08 |
| 19 |
Romans, Mya O (11)
|
NICORY |
42.35 |
43.39 |
1.04 |
| 20 |
Rule, Lilyan M (12)
|
NIUNAC |
48.29 |
45.04 |
-3.25 |
| 21 |
Richeda, Olivia J (11)
|
NIUNAC |
52.67 |
48.16 |
-4.51 |
| 22 |
Michel, Georgia G (11)
|
NIGGST |
47.37 |
48.51 |
1.14 |
| 23 |
Callaghan, Kelsey J (12)
|
NIVEST |
|
49.25 |
- |
| 24 |
Fullmer, Natalie M (12)
|
NIKRKN |
1:12.52 |
50.19 |
-22.33 |
| 25 |
Perry, Teegan R (11)
|
NIKVAC |
|
50.36 |
- |
| 26 |
Wood, Adyson N (12)
|
NIKRKN |
1:00.21 |
51.35 |
-8.86 |
| 27 |
Boyle, Elizabeth E (12)
|
NIWAAC |
|
56.27 |
- |
|
Wilson, Brenna M (11)
|
NIKRKN |
|
DQ |
- |